在RNA和前病毒性DNA基因定型中检测HIV-1耐药性的检测在纵向和重复测试期间都是可变的
Michelle L D'Antoni1, Kristen Andreatta1, Silvia Chang1
1Virology, Gilead Sciences, Inc., Foster City, CA, USA.
概括
随着时间的推移,HIV-1的抗性相关突变 (RAMs) 并非总是被始终地检测到. 了解个体的累积抗药史对于有效的艾滋病毒治疗管理至关重要.
科学领域:
- 病毒学 病毒学
- 遗传学 是一个遗传学.
- 传染性疾病 传染性疾病
背景情况:
- 具有抗性相关突变 (RAMs) 的HIV-1变种可以在潜伏储存中持续存在.
- 了解RAMs的长期持久性是有限的,因为水库动态和测试检测限制.
研究的目的:
- 研究艾滋病毒成年人的RAM检测模式.
- 分析RAM检测在HIV-1病毒储库中的纵向持久性和可重现性.
主要方法:
- 来自比克特格拉维尔/埃姆特里西塔宾/特诺福维尔阿拉芬胺转换研究的基因型数据的回顾性分析.
- 从具有多个切换前时间点的参与者中对RNA和前病毒DNA基因型的纵向分析.
- 将RAM检测分类为100%或50%的检测,并评估随着时间的推移的持久性,损失,增益或不一致性.
主要成果:
- 在两个时间点追踪的262个RAM中,39%显示100%的检测,61%显示50%的检测.
- 在具有≥3个时间点的参与者中,RAM检测是持久的 (19%),丢失的 (12%),获得的 (26%) 或不一致的 (42%).
- 预病毒RAM检测的平均可重复性高 (80.9%),但可变.
结论:
- 没有观察到RAMs的一致的纵向检测模式.
- 普罗维拉尔基因型定型显示出高但可变的可复制性.
- 由于RAM检测不一致,累积抗药史对于最佳的HIV治疗管理至关重要.
相关概念视频
01:10Retrovirus Life Cycles
49.1K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
49.1K
00:36Viral Mutations
39.5K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
39.5K