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cAMP-CRP通过对hfqq的转录抑制来降低RNAI稳定性,促进ColE1等离子体的复制

Qi Zhou¹, Na Li¹, Lingling Jiao¹

¹College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China.

Biochemical and biophysical research communications

|September 20, 2025

概括

循环AMP受体蛋白 (cAMP-CRP) 通过减少Hfq表达来增强ColE1等离子体复制,从而稳定RNAI. 这种机制对于抗生素耐药性基因在肠道细菌中传播至关重要,突出显示了一个保存的调节轴.

科学领域:

微生物学微生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

质粒是抗生素耐药性基因 (ARG) 的关键载体.
在肠杆菌中常见的COLE1型等离子体携带ARG,并依赖RNAI,RNAII和cAMP-CRP进行复制控制.
Hfq,一个RNA伴侣,稳定RNAI以抑制复制,而cAMP-CRP抑制Hfq表达,但直接联系不清楚.

研究的目的:

为了阐明RNAI,RNAII和cAMP-CRP在ColE1等离子体复制中的精确相互作用.
通过Hfq.调查cAMP-CRP在等离子体复制中的调节作用.
为了确定cAMP-CRP-Hfq-RNAI调节轴是否在肠杆菌中保持.

主要方法:

评估不同碳来源 (葡萄糖与糖醇) 和cAMP水平下的ColE1等离子体复制.
分析野生型和突变菌株 (Δhfq,crp,cyaA) 的等离子体产量.
通过Hfq表达水平调查cAMP-CRP对RNAI稳定性的影响.

主要成果:

糖醇支持比葡萄糖更高的ColE1等离子体复制;cAMP的添加逆转了葡萄糖的抑制.
cAMP-CRP刺激ColE1等离子体复制,主要通过Hfq,因为crp或cyaA的突变在Δhfq突变者中没有进一步的影响.
cAMP-CRP抑制hfq表达,降低RNAI稳定性,从而促进等离子体复制.
这种cAMP-CRP-Hfq-RNAI调节机制在肠杆菌中的ColE1等离子体中得到保护.

结论:

cAMP-CRP通过降低Hfq的调节促进ColE1等离子体的复制,导致RNAI稳定性降低.
这项研究揭示了Enterobacterales中等离子体复制的新型调节机制.
了解这种保存轴为打击抗生素耐药性基因传播提供了潜在的策略.

关键词:

抗生素耐药性基因是抗生素耐药性的基因.在ColE1等离子体复制过程中,这就是Hfqq.在RNAI稳定性方面.在cAMP-CRP中使用.

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