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UL24基因影响瘟病毒的复制,其删除减弱毒性,使其可以作为疫苗使用

Huijun Cao¹, Peilin Ruan¹, Hongying Chen¹

¹Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education of the People's Republic of China, International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Research Center of Avian Disease and Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Poultry science

|September 20, 2025

概括

瘟疫病毒 (DPV) UL24蛋白对病毒复制至关重要. 删除UL24基因创造了一个安全,有效的DPV疫苗候选人,对毒性菌株提供100%的保护.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
兽医医学兽医医学兽医医学

背景情况:

瘟疫病毒 (DPV) 在产业造成了重大经济损失.
DPV UL24蛋白 (pUL24) 的功能在很大程度上是未知的.
了解pUL24是制定有效的DPV控制策略的关键.

研究的目的:

为了研究DPV UL24蛋白的功能和致病作用.
评估UL24删除DPV菌株作为减弱疫苗的潜力.

主要方法:

免疫光测试以确定pUL24在子胚胎纤维细胞中的定位.
UL24删除和NLS动机删除DPV菌株的构建和表征.
对病毒复制的评估,子中的毒性,以及删除菌株的保护功效.

主要成果:

pUL24定位到细胞核和细胞核,包含核定位信号 (NLS) 和核定位信号 (NoLS).
删除UL24基因显著降低了子中的DPV毒性.
UL24删除菌株 (DPV-UL24null) 显示出遗传稳定性和免疫性.
用DPV-UL24null免疫的子100%免受病毒性DPV菌株的挑战.

结论:

DPV UL24基因在病毒复制和发病过程中起着重要作用.
由于其安全性和有效性,DPV-UL24null菌株是减弱瘟疫苗的有希望的候选人.

关键词:

瘟疫病毒病毒的感染.免疫保护免疫保护免疫保护核定位信号是一个核定位信号.复制复制复制复制复制复制复制 UL24 UL24 UL24 UL24 UL24 UL24 UL24 UL24 UL24 UL24 UL24 UL24

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