来自Physalis pubescens L.茎的多糖的结构特征,以及它们的神经保护作用和抗衰老活动
Ke Zhou1, Xingyue Zhang1, Xuegui Liu2
1College of Chemical Engineering, Shenyang University of Chemical Technology, Shenyang, 110142, PR China.
International journal of biological macromolecules
|September 20, 2025
概括
从Physalis pubescens L.茎中分离出了两种新型酸性多糖,即PPLS-1和PPLS-2,显示出显著的抗氧化和神经保护作用. 这些化合物通过调节关键细胞通路并延长寿命,显示出作为天然抗衰老剂的潜力.
科学领域:
- 植物化学 植物化学
- 生物化学 生化学
- 老年学是指老年学的学科.
背景情况:
- 青春期植物 (Physalis pubescens L.) 是一种经济性作物,有可能发现新的生物活性化合物.
- 酸性多糖是众所周知的多种生物活性,包括抗氧化和免疫调节作用.
- 氧化应激与衰老和神经退行性疾病有关,需要寻找有效的抗氧化剂.
研究的目的:
- 从Physalis pubescens L.干中分离和描述新的酸性多糖体.
- 评估这些孤立的多糖的抗氧化,神经保护和抗衰老潜力.
- 阐明它们生物活动的潜在分子机制.
主要方法:
- 使用 DEAE-52 和 Sephadex G-200 列色谱分离和净化多糖类.
- 通过分子量测定,单糖组成分析和核磁共振 (NMR) 光谱学进行结构性表征.
- 在体外抗氧化剂测定 (DPPH,ABTS),细胞保护测定 (SH-SY5Y细胞),西部斑点分析,以及在体内对Drosophila melanogaster的寿命研究.
主要成果:
- 两种新型的酸性多糖,PPLS-1 (12.7 kDa) 和PPLS-2 (11.6 kDa),成功地被分离和表征.
- 无论是PPLS-1还是PPLS-2,都表现出显著的DPPH和ABTS激素清除活动,并保护SH-SY5Y细胞免受H2O2诱导的氧化损伤.
- PPLS-1通过降低MDA,增强SOD,T-AOC和GSH-Px活动以及调节Keap1-Nrf2/HO-1通路来显示神经保护作用. 它还延长了Drosophila melanogaster的寿命.
- 果中PPLS-1的寿命延长与Nrf2和HO-1mRNA水平的上调有关.
结论:
- PPLS-1和PPLS-2是来自Physalis pubescens L.茎的新型酸性多糖体,具有强大的抗氧化和神经保护性质.
- PPLS-1有效缓解氧化应激并延长寿命,可能通过Keap1-Nrf2/HO-1信号通路.
- 这些发现突出了Physalis pubescens L.多糖的潜力,作为抗氧化和抗衰老应用的功能成分.
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