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在尼罗河鱼 (Oreochromis niloticus) 中补体受体3 (CR3) 的功能性表征:对抗细菌感染的CD11b/CD18介导免疫力的洞察

Yang Lei¹, Weiheng Shi¹, Yanxi Guo¹

¹Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Engineering Technology Research Center for Environmentally-Friendly Aquaculture, School of Life Sciences, South China Normal University, Guangzhou, 510631, PR China.

Developmental and comparative immunology

|September 20, 2025

概括

研究人员在尼罗河鱼中确定了关键的免疫蛋白,即OnCD11b和OnCD18. 这些蛋白质对于识别和清除细菌感染,增强鱼类免疫力和提供水产养殖疾病解决方案至关重要.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
水产养殖是水产养殖的一种方式.

背景情况:

补充受体3 (CR3),或整合素αMβ2,对于白细胞功能至关重要.
了解CR3在鱼类免疫力中的作用对于水产养殖健康至关重要.

研究的目的:

在尼罗河鱼 (Oreochromis niloticus) 中克隆和表征CR3的αM (OnCD11b) 和β2 (OnCD18) 子单元.
研究OnCD11b和OnCD18在对细菌病原体的免疫反应中的体内和体外功能.

主要方法:

对OnCD11b和OnCD18的序列分析和结构对齐.
遗传学分析以确定进化关系.
细菌挑战后组织特异性表达概况和上调分析.
在体外结合测定与细菌病原体和LPS.
在体内基因淘汰实验以评估功能角色.

主要成果:

克隆和表征了OnCD11b和OnCD18基因,显示了与其他物种的正义基因具有很高的保护性.
主要表达在头部脏和脏,分别,在Streptococcus agalactiae和Aeromonas hydrophila感染后显著上调.
重组vWFA域的OnCD11b和OnCD18显示了与细菌和LPS的特定结合,作为模式识别受体.
在体内击败OnCD11b或OnCD18导致细菌负载增加,证实了它们在宿主防御中的重要作用.

结论:

OnCD11b和OnCD18是尼罗河鱼免疫系统的关键组成部分,对细菌清除至关重要.
这些子单元作为模式识别受体,直接与病原体相互作用.
这些发现提供了对保存的CR3介导免疫力和水产养殖细菌感染的潜在治疗点的见解.

关键词:

细菌感染是一种细菌感染.补充受体3 (CR3) 是一个补充受体.豁免权是一种豁免权.色色素尼洛蒂库斯 (Oreochromis niloticus) 是一种多彩的植物.·维尔布兰德因子A型 (vWFA) 域

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