通过用经过修改的高亲和度阿普坦米尔制成的电化学传感器检测马拉绿色
Zanlin Chen1, Haiming Li1, Yian Chen2
1Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Mikrochimica acta
|September 21, 2025
概括
研究人员开发了一种新的电化学生物传感器,使用高亲和度的吸收体来检测食品样本中的马拉绿 (MG). 这种先进的aptamer生物传感器提供了高灵敏度和特异性,以改善食品安全监测.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 马拉绿 (MG) 是一种在水产养殖中使用的合成染料,具有潜在的健康风险.
- 准确和敏感的MG检测方法对于确保食品安全至关重要.
研究的目的:
- 开发一个高亲和度的aptamer用于马拉绿色检测.
- 为敏感和特定的MG量化建立一个电化学的aptamer生物传感器.
主要方法:
- 分子对接被用来设计和优化体.
- 通过位点定向突变发生生成了一种高亲和度的阿胺体 (MG-15-GGT).
- 一个电化学生物传感器是使用修改的aptamer构建的.
主要成果:
- 优化的阿普坦 (MG-15-GGT) 呈现出2.71 ± 1.95μM的低解离常数 (Kd).
- 电化学阿普坦生物传感器实现了2.74 pM的检测极限 (LOD).
- 传感器表现出极好的特异性和恢复率 (87.1111.5%).
结论:
- 修改后的阿普坦可以显著提高生物传感器检测污染物的性能.
- 开发的电化学阿普坦生物传感器是食品安全应用中马拉绿色监测的一个有前途的工具.
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