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使用自主氨酸生物合成的工程大肠杆菌 (Escherichia coli) 进行经济高效的黑色素生产

Ling Zou¹, Jian Liu², Yidan Hu²

¹Key Laboratory of Metabolic Engineering and Biosynthesis Technology, Ministry of Industry and Information Technology, Nanjing University of Science and Technology, Nanjing, China.

Journal of biotechnology

|September 21, 2025

概括

这项研究通过启用自主氨酸合成,对大肠杆菌进行了高成本有效的微生物黑色素生产的工程设计. 这种方法可以显著降低生产成本,而不需要外部氨酸补充剂.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
微生物颜料的生产方法

背景情况:

传统的微生物黑色素生产是昂贵的,因为它依赖于昂贵的氨酸补充剂.
开发成本效益高的黑色素合成方法对于工业应用至关重要.

研究的目的:

设计一种大肠杆菌菌株用于自主氨酸合成,以降低黑色素生产成本.
建立一个可持续且具有成本效益的微生物黑色素生产平台.

主要方法:

具有反抗性的共过度表达的氨酸合成酶基因 (aroGfbr或tyrAfbr) 和大肠杆菌中的氨酸酶基因 (tyr1).
将铁酶基因 (tyr1) 集成到用于黑色素生物合成的工程菌株中.
在没有外源性氨酸的情况下培养了工程E. coli菌株.

主要成果:

在没有外源性氨酸的情况下,达到5.58至7.57毫克/毫升的黑色素产量.
与需要补充氨酸的传统方法相比,生产成本降低了70%-73.33%.
证明了自主氨酸合成,使成本效益高的黑色素产生.

结论:

成功设计了大肠杆菌以实现可持续和成本效益的微生物黑色素生产.
开发的平台对微生物颜料的工业制造具有重大影响.
自主氨酸合成是一种可行的策略,可以克服微生物色素生产中的基质依赖.

关键词:

自主氨酸生物合成人工合成的大肠杆菌埃舍里奇亚微生物的黑色素生产.合成生物学合成生物学

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