典型的兽医抗生素和除草剂对Cu/Zn超氧化物脱酶活性的对抗作用:在二次接口上具有竞争性的结合
Liang Tang1, Shiwan Zhang1, Zhenheng Long1
1Key Laboratory of Organic Compound Pollution Control Engineering (MOE), School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
International journal of biological macromolecules
|September 21, 2025
概括
兽医抗生素如硫法迪亚和除草剂如甲米特龙可以破坏牲畜中的抗氧化酶. 这项研究表明,它们在SOD上竞争性地与同一位点结合,对抗性地抑制其活性.
科学领域:
- 环境毒理学环境毒理学
- 生物化学 生化学
- 农业化学安全 农业化学安全
背景情况:
- 牲畜可以同时从料中摄取兽医抗生素和除草剂.
- 对这些农业化学品的联合暴露可能会扰乱 redox 稳态.
- 关于联合农业化学品对抗氧化酶的分子水平影响的数据有限.
研究的目的:
- 为了研究硫法迪亚 (SD) 和甲米特龙 (MET) 对超氧化物脱酶 (SOD) 的联合毒性作用.
- 阐明SD,MET和SOD之间的相互作用背后的分子机制.
- 建立一个框架来评估混合农业化学品的联合毒性影响.
主要方法:
- 多光谱技术 多光谱技术
- 在X射线光电子光谱学 (XPS) 中.
- 分子对接是分子对接.
- 光灭分析的分析.
主要成果:
- SOD无活化是SD和MET在关键地点直接结合的结果.
- SD和MET表现出与SOD结合的1:1结合静脉测量,表明一个单一的偏好的结合部位.
- 这些化学物质通过结合到SOD跨子单元腔内的相同位置来竞争性地抑制SOD活性.
- 谷氨酸,氨酸和氨酸残留物被确定为关键相互作用地点.
结论:
- 开发了一种新的"相互作用-微环境-构造"框架,以评估关节农业化学毒性.
- 这项研究为SOD-农业化学相互作用提供了分子层面的理解.
- 这项研究提供了一种新的方法来评估混合农业化学品暴露在牲畜中的健康风险.
相关概念视频
Combined Effects of Drugs: Synergism
6.8K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
Such synergistic combinations...
6.8K
Combined Effects of Drugs: Antagonism
11.6K
The combined effects of drugs can result in various interactions, of which an important type is antagonism. Antagonism is a mechanism where one drug inhibits or counteracts the effects of another drug. Antagonism can occur through various means, including receptor binding, allosteric modulation, functional interaction, chemical reactions, and pharmacokinetic processes.
The most common type is receptor antagonism, where one drug acts as an antagonist to block the effects of another drug by...
The most common type is receptor antagonism, where one drug acts as an antagonist to block the effects of another drug by...
11.6K
The Electron Transport Chain
19.7K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
19.7K
Gene Regulation in Microbial Communities: Quorum Sensing
533
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
533
Factors Affecting Protein-Drug Binding: Drug Interactions
561
Drug interactions are a critical aspect of pharmacology and can occur when two or more drugs compete for the same binding site. This competition can result in one drug displacing another, altering the effect of the displaced drug. Drug interactions are complex processes that rely heavily on how much of the displacer drug is present and how strongly it can bind to the same sites as the displaced drug.
Displacement interactions can have varying outcomes, ranging from toxicity to virtually...
Displacement interactions can have varying outcomes, ranging from toxicity to virtually...
561
Antibiotic Selection
59.4K
Overview
59.4K


