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Updated: Jan 17, 2026

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Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
Published on: September 19, 2017
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沉重稀土元素诱导的G-四复合DNA的分子机制:人类端粒变体中的结构切换
Sampat N Satapathy1, Umakanta Subudhi1
1DNA Nanomaterials & Application Laboratory, Environment & Sustainability Dept., CSIR-Institute of Minerals & Materials Technology, Bhubaneswar 751013, India; Academy of Scientific & Innovative Research (AcSIR), Faculty of Biological Sciences, Ghaziabad, 201002, India.
International journal of biological macromolecules
|September 21, 2025
概括
重稀土元素 (HREEs) 可以在人类端粒DNA中形成稳定的G-四重复合体 (GQs). 这种相互作用会影响基因组的稳定性,并对环境毒性和生物感知应用产生影响.
科学领域:
- 生物化学 生物化学
- 基因组学就是基因组学.
- 环境科学 环境科学
背景情况:
- 重稀土元素 (HREEs) 对技术至关重要,但它们的生物相互作用尚不清楚.
- 由于与DNA的潜在相互作用,对HREE的环境暴露引起了对基因组稳定性的担忧.
- 端粒DNA结构特别脆弱,它们由HREEs的调制需要研究.
研究的目的:
- 研究HREEs与人类端粒DNA及其变体的相互作用.
- 为了确定HREE-DNA复合体的结构和热力学特征.
- 阐明HREE结合对端粒DNA构造和稳定性的影响.
主要方法:
- 微分子度的HREEs被用来诱导G-四重复 (GQ) 在端粒DNA的形成.
- 使用形态,热和H NMR光谱学来描述GQ结构和稳定性.
- 进行了热力学表征,泽塔潜力分析和竞争性结合研究,以评估结合亲和力,固态度和离子位移.
主要成果:
- 在人类端粒DNA及其变体中,HREEs诱导了稳定,反平行的GQs.
- GQ稳定性显示出依赖序列的差异,而富含胺的循环增强了稳定性.
- 1H NMR证实了Hoogsteen的键,热力学研究显示了2:1的HREE-DNA静态测量.
- 结合HREE改变了静电电荷分布,并将Na+/K+离子移位,诱导了形状转换.
结论:
- HREEs可以与端粒DNA形成稳定的G-四重复结构,从而影响其构造和稳定性.
- 这些发现凸显了环境HREE暴露对基因组稳定性的潜在风险.
- 这项研究提供了与端粒生物学,毒性和基于核酸的生物传感相关的HREE-DNA相互作用的见解.
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