在inocybe和psilocybe中进行psilocybin生物合成的不同反应和酶
Tim Schäfer1,2, Fabian Haun1,2, Bernhard Rupp3,4
1Pharmaceutical Microbiology, Friedrich Schiller University, Winzerlaer Str. 2, 07745, Jena, Germany.
Angewandte Chemie (International ed. in English)
|September 22, 2025
概括
独立地进化了产生 псилоцибин (4-氧化-N,N-二甲基三胺) 的能力. 不同于Psilocybe属,Inocybe属使用不同的酶,导致psilocybin和baeocystin,不同于Psilocybe属.
科学领域:
- 生物化学 生物化学
- 菌类学 菌类学是指菌类学.
- 代谢工程是代谢工程.
背景情况:
- псилоцибин是一种主要的精神活性化合物,存在于许多真菌物种中.
- 在Psilocybe中psilocybin的生物合成是众所周知的,从l-tryptophan开始.
- 一些Inocybe物种,如Inocybe corydalina,也产生了psilocybin,但它们的生物合成途径是未知的.
研究的目的:
- 为了阐明在Inocybe corydalina中psilocybin的生物合成途径.
- 为了比较Psilocybe和Inocybe世代之间的psilocybin生产的酶机制.
- 了解真菌中 псилоцибин生物合成的独立进化.
主要方法:
- 来自Inocybe corydalina的四种关键酶 (IpsD,IpsK,IpsM1,IpsM2) 的重组表达和体外表征.
- 在单氧化酶 (IpsH) 的基分析.
- 在Inocybe和Psilocybe物种之间对酶途径的比较分析.
主要成果:
- 在Inocybe corydalina中鉴定和描述了四种参与psilocybin生物合成的酶.
- 证明Inocybe corydalina与Psilocybe物种相比使用了一组不同的酶.
- 揭示了Inocybe中分支的途径,导致psilocybin和baeocystin,没有与Psilocybe途径共享的反应.
- 表明IpsD作为l-三甲酸脱碳酶,IpsK作为激酶,IpsM1/IpsM2作为甲基转移酶.
结论:
- 菌物种已经独立地使用不同的酶组进化了对psilocybin生物合成的代谢能力.
- 伊诺西贝途径代表了新的途径,以psilocybin和 baeocystin,突出突出融合进化.
- 从无关的路径中招募酶使得在不同的真菌系中可独立发展psilocybin生产.
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