在炎症性肠病队列中开发和临床评估使用超高通量PRM-MS的多重健康监测小组
Qin Fu1, Philip M Remes2, Jihyeon Lee1
1Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Angewandte Chemie (International ed. in English)
|September 22, 2025
概括
本研究提出了一种强大的多重并行反应监测 (PRM) 试验,用于定量57种血蛋白,在大型队列中证明生物标志物验证的高准确性和可重复性. 该试验成功验证了炎症性肠病 (IBD) 候选生物标志物.
科学领域:
- 临床蛋白质组学 临床蛋白质组学
- 生物标志物的发现和验证.
- 基于质谱学的测试.
背景情况:
- 将蛋白质生物标志物发现转化为临床实践受到复杂的验证过程的阻碍.
- 针对性质谱 (MS) 测定,与并行反应监测 (PRM) 一样,对于敏感和特定的生物标志物翻译至关重要.
研究的目的:
- 开发和验证用于量化血蛋白质的多重PRM试验.
- 为了评估测试在各种样本输出中的性能.
- 为了验证炎症性肠病 (IBD) 的候选生物标志物.
主要方法:
- 在恒星质谱平台上开发多重PRM试验,以量化57种血蛋白.
- 测试参数的优化和不同样本吞吐量 (100-300个样本/天) 的负载曲线的评估.
- 对493名IBD患者和509名对照患者的大队伍的分析,包括系统适应性测试和质量控制.
主要成果:
- PRM测试显示了高量化性,包括线性,灵敏度和可重复性.
- 在大IBD队列中实现了一致的数据采集.
- 候选IBD生物标志物,C-反应蛋白和orosomucoid蛋白被成功验证.
结论:
- 开发的多重PRM试验适用于高通量,准确量化血蛋白质用于生物标志物验证.
- 这种方法有助于将蛋白质组的发现转化为临床应用.
- 该研究成功验证了炎症性肠病的关键生物标志物.
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