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在芯片上使用振动诱导的流量去除卵细胞积累.

Amirhossein Favakeh1, Amir Mokhtare1,2, Hanxue Zhang3

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对于辅助生殖技术 (ART) 至关重要的累积去除 (CR) 现在是自动化的. 使用振动诱导流 (VIF) 的新平台有效地从卵细胞中去除累积细胞,确保在不影响受精率的情况下获得一致的结果.

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科学领域:

  • 生物技术是生物技术.
  • 生殖生物学 生殖生物学
  • 生物工程是生物工程.

背景情况:

  • 累积去除 (CR) 是辅助生殖技术 (ART) 中的一个关键步骤.
  • 传统的CR方法,如手动管道,可以引入变化和不一致性.
  • 在ART中需要自动化和标准化CR技术.

研究的目的:

  • 开发和评估一个开放的表面平台,用于从卵细胞中自动去除累积物 (CR).
  • 为了研究振动诱导流 (VIF) 对CR的有效性.
  • 为了比较基于VIF的CR与传统手动方法在卵细胞存活和发育潜力的表现.

主要方法:

  • 使用柱子和振动诱导流 (VIF) 的开放表面平台被设计用于颗粒和累积细胞分离.
  • 通过调整启动功率,时间和 hialuronidase (HA) 度来优化 CR 过程.
  • 小鼠累积卵细胞复合体 (COCs) 通过VIF或手动管道进行CR,然后进行授精和评估受精率和胚胎细胞率.

主要成果:

  • 该VIF平台证明了小颗粒的有效分离,从装载室中去除了99%.
  • 在没有任何卵细胞损失的情况下,达到最多23个卵细胞的同时CR.
  • 在VIF和手动CR方法之间没有观察到受精 (90.7%手动vs. 93.1%VIF) 或胚胎囊率 (50.0%手动vs. 43.1%VIF) 的统计学上显著差异.

结论:

  • 开发的VIF平台有效地以高通量方式自动化累积去除 (CR).
  • 这项技术减少了人工劳动,并提高了辅助生殖技术 (ART) 的标准化.
  • 基于VIF的CR是一种可行的手工方法的替代方案,可以保持卵细胞质量和发育潜力.