在性结肠炎中,β-葡萄糖和Lactobacillus johnsonii NSP009的协同保护作用
Shanshan Zhang1, Yonggan Sun1, Linlin Gao1
1State Key Laboratory of Food Science and Resources, China-Canada Joint Lab of Food Science and Technology, Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, Nanchang, 330047, China. spnie@ncu.edu.cn.
一种结合β-葡萄糖 (BG) 和Lactobacillus johnsonii (LJ) 的新型共生菌在性结肠炎 (UC) 鼠标模型中有效降低了炎症并改善了肠道屏障功能. 这种β-葡萄糖和Lactobacillus johnsonii (BGLJ) 配方对管理UC有希望.
科学领域:
- 胃肠病学 胃肠病学
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性肠病.
- 益生菌和益生菌显示出对UC的治疗潜力.
- 贝塔葡萄糖 (BG) 和牛乳杆菌 (LJ) 单独表现出抗结肠炎作用.
研究的目的:
- 为了研究一种新型共生菌配方BGLJ (BG和LJ NSP009) 的治疗效果,在硫酸 (DSS) 诱导的大肠炎小鼠模型中.
- 探索BGLJ保护作用的潜在机制.
主要方法:
- 在小鼠中使用DSS诱导大肠炎.
- 用BGLJ共生配方的管理.
- 评估疾病活动指数,减肥,肠道炎症和屏障功能障碍.
- 量化L. johnsonii的丰富度和英多尔-3-乳酸 (ILA) 的水平.
- 评估基碳化合物受体 (AhR) 激活和IL-22的产生.
主要成果:
- BGLJ显著缓解了DSS诱导的体重减轻,并降低了疾病活性指数.
- BGLJ缓解了肠道炎症和改善了屏障功能.
- BGLJ治疗增加了L. johnsonii的丰富性和印-3-乳酸 (ILA) 的产量.
- BGLJ提高了肠道ILA水平,导致AhR激活并增加了IL-22的产生.
结论:
- BGLJ同生菌配方显示出显著的保护作用,对DSS诱导的大肠炎在小鼠.
- BGLJ通过增加L. johnsonii,ILA生产,AhR激活和IL-22水平来促进肠道平衡.
- BGLJ代表了一种有前途的新型共生干预,用于治疗性结肠炎.
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