在双相氨酸填充剂中通过非交叉连接相调节质性质
Orhan Gokalp Buyukuysal1, Zeynep Caglar1, Alkin Ozgen1
1Institute of Science, Bioengineering Division, Hacettepe University, Beytepe, Ankara 06800, Turkey.
ACS omega
|September 22, 2025
概括
这项研究开发了更安全的氨酸 (HA) 皮肤填充剂,使用绿色交叉链接剂酸 (CA). 最佳的双相HA填充剂配方显示出出色的生物相容性和用于软组织增强的机械性能.
科学领域:
- 生物材料科学 生物材料科学
- 皮肤填充剂 皮肤填充剂
- 组织工程是组织工程.
背景情况:
- 基于氨酸 (HA) 的软组织增强材料广泛用于化品和重建目的.
- 目前的HA填充剂经常使用可构成毒性风险的交叉链接器.
- 需要更安全,更有效的皮肤填充材料,具有可调节的机械性能.
研究的目的:
- 用酸 (CA) 作为绿色交叉链接剂合成新的双相氨酸 (HA) 皮肤填充剂.
- 评估开发的HA填充剂的机械,学和生物相容性特性.
- 为了确定一个最佳的HA填充剂配方用于软组织增强.
主要方法:
- 双相HA填充剂是通过将非交叉链接的HA与不同度的CA (4-12%w/w) 以及使用1,4-Butanediol diglycidyl ether (BDDE) 合成的交叉链接的HA相结合而制备的.
- 使用蒸汽灭菌,并评估其对物理性能的影响.
- 测量了风湿学特性 (G',G′′, η*,相角),并使用使用L929细胞的MTT测定来评估细胞活力.
- 在体内生物相容性根据ISO 10993标准进行了评估,包括组织病理学评估.
主要成果:
- 含有10%的CA的BP-E-10-S样品被确定为最佳配方.
- 这种最佳样本表现出有利的风学特性:弹性模量 (G') 为388.76 ± 13.91Pa,损耗模量 (G′′) 为88.89 ± 6.22Pa,复合粘度 (η*) 为12.69 ± 0.47Pa·s,相角为12.86 ± 0.45°.
- 蒸汽灭菌没有产生有毒废物.
- 在体内研究证实BP-E-10-S配方是无刺激性和生物相容的 (ISO 10993-6:2016,得分1.92).
- 开发的配方获得了专利.
结论:
- 酸 (CA) 作为一种有效且更安全的绿色交叉链接剂,用于合成双相酸 (HA) 皮肤填充剂.
- 开发的双相HA填充剂具有可调节的机械性能,并显示出出色的生物相容性,使它们适合软组织增强.
- 最佳的BP-E-10-S配方代表了皮肤填充剂领域的有希望的进步,提供了更高的安全性和有效性.
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