广泛的基质特异性和高催化活性的Sphingomonadaceae的PhoK型酸酶涉及阻燃降解
Landry Freeman1, Andrew Davis1, Harley Gossen1
1Department of Chemistry and Physics, Southwestern Oklahoma State University, Weatherford, Oklahoma 73096, United States.
ACS omega
|September 22, 2025
概括
来自Sphingomonadaceae的PhoK型酸酶表现出高酶效率和广泛的基质特异性. 这些酶非常适合用于生物修复和工业应用,需要高周转率.
科学领域:
- 生物化学和环境微生物学
- 酵素学和生物催化剂学
背景情况:
- 属于核酸铁酸酶/化酶 (NPP) 家族的PhoK型酸酶具有进化的酸酶活性.
- 关于Sphingobium sp.的初步研究 它们包括TCM1 (Sb-PhoK) 和Sphingomonas sp. BASR1 (Sm-PhoK) 突出了它们在生物修复中的作用.
- 关于这些酶与环境体和阻燃衍生物的基质特异性和活性的知识有限.
研究的目的:
- 为了识别和描述来自Sphingomonadaceae家族的Phok同类.
- 调查这些酶的基质特异性和运动性质,针对各种各样的有机酸单和酸.
- 评估Sphingomonadaceae PhoK酶在生物修复和工业应用中的潜力.
主要方法:
- 生物信息学分析被用来识别Sphingomonadaceae家族中的Phok同类.
- 九个被选中的同类体经历了动力学表征.
- 合成的有机酸单 (来自阻燃剂),环境酸和生物单酸的图书馆被用作基质.
主要成果:
- Sphingomonadaceae PhoK酶在广泛的基质中表现出高的酶效率.
- Sm-PhoK对基酸具有显著的活性 (kcat=1100 s−1,kcat/Km=1.8 × 106 M−1 s−1).
- 来自Sphingobium yanoikuyae (Sy-PhoK) 的同类显示出最高的整体活性,化所有测试的基质,效率>3 × 104 M-1 s-1.1.
结论:
- 来自Sphingomonadaceae的特征Phok酶具有广泛的基质特异性和高的催化活性.
- 这些酶性质使它们成为生物修复有机酸盐污染物的非常适合的候选者.
- 它们的高周转率也使它们在各种商业生物催化剂应用中具有优势.
相关概念视频
Bacterial Phylum Bacteroidota
591
The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
591
Protein Kinases and Phosphatases
15.0K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
15.0K
Phosphoinositides and PIPs
10.1K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
10.1K
Bacterial Phylum Planctomycetes
418
Planctomycetes are a group of morphologically distinct bacteria predominantly classified into two orders: Planctomycetales and Brocadiales. These gram-negative bacteria exhibit unique features, including division by budding and the presence of stalks or appendages. Their cells are often found in rosette arrangements, and they are notable for possessing an S-layer in their cell envelope, which is relatively uncommon among bacteria. Additionally, Planctomycetes frequently exhibit intracellular...
418
Gene Regulation in Microbial Communities: Quorum Sensing
533
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
533
Amplifying Signals via Enzymatic Cascade
17.5K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
17.5K


