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口腔病原体对口腔细胞中炎症媒介表达的影响

Pratik Sanjay Hodar¹, Mitesh Shah², Yihan Fu³

¹Department of Prosthodontics & Crown and Bridge, Shri Yashwantrao Chavan Memorial Medical & Rural Development Foundation's Dental College & Hospital, Vadgaon Gupta Road, Ahmednagar - 414003, Maharashtra, India.

|September 22, 2025

概括

一些口腔细菌,如 Porphyromonas gingivalis,显著增加了口腔细胞中的炎症性细胞因子. 这表明这些病原体在启动口腔炎症方面发挥了作用,并突出了针对性微生物疗法的潜在益处.

科学领域:

口腔微生物学口腔微生物学
免疫学免疫学免疫学

背景情况:

口腔病原体,包括 Porphyromonas gingivalis (P. gingivalis),Fusobacterium nucleatum (F. nucleatum) 和Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans),都与各种口腔疾病有关.
细胞因子在口腔组织内调解炎症反应中起着至关重要的作用.

研究的目的:

调查关键口腔病原体对口腔上皮质细胞中促炎性细胞因子表达的影响.
要量化由P. gingivalis,F. nucleatum和A. actinomycetemcomitans引起的特定细胞因子水平的增加.

主要方法:

使用定量逆转录聚合酶链反应 (qRT-PCR) 来测量细胞因子基因表达.
口腔上皮细胞被暴露在P. gingivalis,F. nucleatum和A. actinomycetemcomitans. 的作用下.

主要成果:

在24小时后,P. gingivalis显著增加了4.2倍的白蛋白-1β (IL-1β),3.8倍的瘤缩因子α (TNF-α) 和3.5倍的白蛋白-6 (IL-6).
F. nucleatum和A. actinomycetemcomitans也诱导了细胞因子水平的显著增加,从2.4倍到3.6倍 (p <0.05).
这些发现表明,这些口腔病原体的存在与促炎途径的激活之间存在明显的联系.

结论:

口腔病原体P. gingivalis,F. nucleatum和A. actinomycetemcomitans是口腔上皮细胞中促炎性细胞因子的强有力的诱导剂.
这些细胞因子由特定的口腔细菌激活,有助于口腔炎的开始和进展.
向微生物疗法作为治疗策略,有望成为管理由这些病原体驱动的口腔炎症状况的治疗策略.

关键词:

聚合菌是Actinomycetemcomitans的组合体之一.杆菌核酸 (Fusobacterium nucleatum) 是一种细菌.口腔病原体是一种口腔病原体.它们是 Porphyromonas gingivalis.细胞因子细胞因子炎症调解者是一种炎症调解者.口腔内皮质细胞是口腔内皮质细胞.牙周炎是指牙周炎的一种疾病.

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