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与精液采集时间和公牛品种相关的细菌概况的变化,使用16S rRNA测序和MALDI-TOF MS进行分析

Aleksandar Cojkic¹, Adnan Niazi^2,3, Ingrid Hansson²

¹Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.

Frontiers in veterinary science

|September 22, 2025

概括

牛精液细菌群落因品种和采集时间而异. 了解这种种子微生物群对于有针对性的抗生素使用和管理抗菌素耐药性 (AMR) 至关重要.

科学领域:

兽医微生物学兽医微生物学
动物繁殖动物繁殖
基因组学就是基因组学.

背景情况:

牛精液中的细菌污染需要使用抗生素,这引发了对抗菌素耐药性 (AMR) 的担忧.
有针对性的抗生素策略需要准确识别种子细菌群落.
以前的研究还没有完全描述公牛精液微生物群的动态.

研究的目的:

为了描述公牛精液中的细菌群体.
根据精液采集时间和品种,调查微生物群的变化.
为在精液保存中明智使用抗生素提供数据.

主要方法:

从三个时间点 (T1,T2,T3) 采集的乳牛 (维京霍尔斯坦和维京红) 的精液样本.
使用矩阵辅助激光吸附/电离-飞行质谱时间 (MALDI-TOF MS) 的细菌鉴定.
通过16S rRNA测序和元基因组分析评估的细菌多样性.

主要成果:

确定了33种细菌物种,具有特定于品种和特定于时间点的分布.
甲基因组分析揭示了种类之间的细菌类和属之间的显著差异.
贝塔多样性分析显示,维京霍尔斯坦的样本有明显的聚类,而维京红色样本的变异性更大.
特定的细菌属显示了时间动态和与其他种类的相关性.

结论:

雄牛的种子微生物群表现出显著的变异,受品种和采集时间的影响.
品种和时间因素影响公牛精液细菌的组成和多样性.
这些发现支持了针对牛精液保存中使用抗生素的量身定制方法的必要性,以对抗AMR.

关键词:

16s 测序的测序马尔迪 TOF-MS-MS 的时间细菌细菌细菌是一种细菌.养殖品种养殖品种公牛的精液是什么?微生物组是一个微生物组.

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