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提取优化和抗氧化多糖的特征从牛肉 (Suillus bovinus) 来

Zhuang Li¹, Shuting Fan¹, Xueqi Wang²

¹College of Life Sciences Hebei Normal University Shijiazhuang People's Republic of China.

Food science & nutrition

|September 22, 2025

概括

研究人员使用Box-Behnken设计优化了从Suillus bovinus中提取原始多糖的提取方法. 在细胞模型中,Y-1 分数显示出显著的抗氧化潜力,表明其作为天然抗氧化剂的承诺.

科学领域:

自然产品化学自然产品化学
食品科学食品科学食品科学
生物化学生物化学

背景情况:

来自食用真菌的多糖类具有各种生物活性.
牛 (Suillus bovinus) 是一种具有潜在健康益处的.

研究的目的:

为了优化从Suillus bovinus中提取原始多糖 (SubPs) 的提取.
为了评估纯化多糖分的抗氧化活性.

主要方法:

盒子-Behnken设计与响应表面方法 (BBD-RSM) 结合用于提取优化.
DEAE-52纤维素和塞法德克斯G-100染色学用于净化.
使用HepG2细胞和生物化学分析进行体外抗氧化剂测定.

主要成果:

优化的提取参数产生了SubPs.
获得了四个同质分数 (S-1,S-2,Y-1,Y-2),其分子重量从47.34到97.07kDa不等.
微分Y-1通过降低活性氧物种 (ROS) 和恶性甲酸 (MDA) 水平,并增强抗氧化酶活性 (SOD,CAT,GSH-Px) 来表现出显著的抗氧化活性.

结论:

这项研究成功优化了从Suillus bovinus中提取多糖的方法.
分数Y-1表现出强大的抗氧化特性,得到了体外基于细胞的测定和结构特征的支持.
牛肉的多糖,特别是Y-1分数,显示出作为健康应用中的天然抗氧化剂的潜力.

关键词:

牛肉 (Suillus bovinus) 是一种动物.抗氧化剂活性抗氧化剂活性聚糖是一种多糖.结构分析是结构分析.

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