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Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots
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目前处理骨质合体移植的技术是可变的:系统性审查.

Michael J Meade1, Kisan Patel2, Nicholas A Sgaglione1,2

  • 1Department of Orthopaedic Surgery, Northwell Health, Queens, New York, U.S.A.

Arthroscopy, sports medicine, and rehabilitation
|September 22, 2025
PubMed
概括

骨质合体移植 (OCA) 的最佳储存方式尚不清楚. 虽然4°C是常见的,但37°C和室温储存显示出对状细胞生存能力的承诺,但储存介质缺乏共识.

科学领域:

  • 整形外科手术 整形外科手术
  • 生物医学工程 生物医学工程
  • 组织工程是组织工程.

背景情况:

  • 骨质合体移植 (OCA) 对于膝盖复苏至关重要.
  • 目前OCA的处理和储存方法缺乏标准化的最佳协议.
  • 了解最佳实践对于成功的临床应用至关重要.

研究的目的:

  • 系统地审查和综合当前关于新鲜冷藏骨髓骨质合体移植 (OCA) 最佳处理和储存方案的证据.
  • 评估研究OCA保存技术的体内和体外研究.
  • 识别有关OCA存储和处理的知识差距.

主要方法:

  • 在遵守PRISMA指南的基础上进行系统审查.
  • 在PubMed,Embase和Scopus数据库中搜索相关的同行评审文章.
  • 包括体内和体外人体研究,不包括评论和非人体研究. 提取的数据包括储存条件,介质和结果.

主要成果:

  • 27项研究符合纳入标准,其中包括8项人体体内研究和19项人体外研究.
  • 4°C是最常见的储存温度 (78.95%),一些研究探讨了37°C和室温.
  • 研究了19种不同的存储介质,其中68.42%使用添加剂;对介质的共识仍然难以达成.

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结论:

  • 4°C的储存是普遍存在的,但可能不是最佳的冠状细胞活力.
  • 替代温度 (37°C,室温) 和特定的保存系统显示出改善状细胞密度的潜力.
  • 需要进一步的研究,以建立对OCA存储介质和协议的共识.