使用空间抵消拉曼光谱成像技术评估不弹性散射测量的深度传感极限的实验方法
Hugo Tavera1,2, Guillaume Sheehy1,2, Patrick Orsini3
1Polytechnique Montreal, Department of Physics Engineering, Montreal, Quebec, Canada.
Journal of biomedical optics
|September 22, 2025
概括
空间偏移拉曼光谱 (SORS) 现在可以预测组织感应深度. 这一进步改善了地下生化检测,用于诸如乳腺癌手术利率评估等应用.
科学领域:
- 频谱学是一种光谱学.
- 生物医学光学 生物医学光学
- 化学分析 化学分析
背景情况:
- 空间偏移拉曼光谱 (SORS) 是一个有前途的技术,用于地下生化分析.
- 在SORS中空间偏移和传感深度之间的关系还不太清楚,这限制了临床应用,例如乳腺癌手术边际评估.
研究的目的:
- 开发一种实验方法,以确定SORS中的空间偏移与采样深度之间的关系.
- 为了能够检测地下生物化学成分,以改善SORS.的临床翻译.
主要方法:
- 使用定制的超光谱线扫描成像系统进行SORS检测.
- 采用了具有不同光学特性和厚度的双层幻影 (PDMS/尼龙).
- 开发了一种使用光谱角度映射仪的技术,根据空间偏移来预测传感深度.
主要成果:
- 通过高达3毫米的PDMS,证明了底层尼龙层的可检测性.
- 通过3毫米的脂肪和2毫米的猪脂肪检测出富含蛋白质的组织.
- 基于顶层光学特性,建立了空间偏移和探测深度之间的相关曲线.
结论:
- 证明了使用双层幻象来将空间偏移与探测深度相关联的可行性.
- 开发的技术可以促进SORS的临床翻译,用于瘤检测和边缘评估.
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