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同样的片段,不同的疾病:在疾病中分析相同的tRNA片段,使用功能和丰度数据库

Adesupo Adetowubo¹, Sathyanarayanan Vaidhyanathan^1,2, Andrey Grigoriev^1,2

¹Department of Biology, Rutgers University, Camden, NJ 08102, USA.

|September 22, 2025

概括

转移RNA衍生碎片 (tRFs) 的序列相同,但在疾病中可能有不同的功能. 基于目标的数据库比基于丰度的数据库更好地预测tRF功能,突出显示了综合数据分析的必要性.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

转移RNA衍生碎片 (tRFs) 是参与基因调节和疾病的小型非编码RNA.
它们的目标特异性和在各种疾病背景中的精确作用尚未完全理解.
在不同疾病中调查相同的tRF序列对于理解它们的功能多样性至关重要.

研究的目的:

探索在不同的疾病环境中出现相同的tRF序列的现象.
评估实验验证的tRF函数与现有数据库中的预测之间的一致性.
评估基于目标的与基于丰度的tRF数据库在预测功能相关性的实用性.

主要方法:

从多种疾病研究中选择了5个具有相同序列的tRF.
从文献中提取了经过验证的目标和疾病关联.
使用tatDB,tRFTar和tsRFun数据库对预测目标进行交叉引用.
在使用TCGA数据的OncotRF和MINTbase中评估了tRF丰富度丰富.

主要成果:

只有一个tRF (LeuAAG-001-N-3p-68-85) 显示实验数据和基于目标的数据库预测之间完全一致.
其他tRF在预测的约束区域与验证的目标中表现出部分重叠.
tRF 丰度数据显示丰度不一致,丰度与实验验证疾病关联之间的一致性有限.
功能相关的tRF通常在仅丰富的数据库中表现不佳.

结论:

这项试点研究表明,相同的tRF序列在各种疾病中可能具有不同的功能.
基于目标的数据库为tRF注释提供了比基于丰度的工具更好的机械洞察力.
集成的数据源对于准确的tRF功能推理至关重要,因为单独的表达数据是不够的.

关键词:

阿尔戈诺特人是一个阿尔戈诺特人.这是一次碰撞,碰撞.清除清除清除在TCGA中,TCGA就是TCGA.癌症癌症癌症癌症癌症胃癌胃癌是一种胃癌.基因调节基因调节基因调节质母细胞瘤是一种质母细胞瘤.胰腺腺癌的癌症.在tRF数据库中.由tRNA衍生的片段.目标预测目标预测

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