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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

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Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping
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在非线性显微镜中进行空间光调节器的直线校准.

Daniël W S Cox, Harish Sasikumar, Ivo M Vellekoop

    Applied optics
    |September 22, 2025
    PubMed
    概括

    我们开发了一种新的校准方法,用于非线性显微镜中仅相位空间光调节器. 该技术使用显微镜图像,不需要额外的硬件,在低光条件下工作良好.

    科学领域:

    • 光学和光子学 在光学和光子学.
    • 显微镜的使用方法
    • 生物物理学的生物物理.

    背景情况:

    • 仅相位空间光调制器 (SLM) 对于先进的显微镜技术至关重要.
    • 精确的SLM校准对于精确控制光波前线至关重要.
    • 现有的校准方法往往需要专门的硬件或复杂的程序.

    研究的目的:

    • 提出一种新的,硬件独立的方法,用于校准非线性显微镜中仅相位的SLM.
    • 为了使SLM校准适应在多光子激发光显微镜中产生的特定非线性信号.
    • 在具有挑战性的成像条件下证明该方法的稳定性.

    主要方法:

    • 拟议的方法使用显微镜自己的图像作为校准测量.
    • 它直接分析多光子激发过程中产生的非线性信号.
    • 校准不需要额外的光学组件或外部硬件.

    主要成果:

    • 该方法在非线性显微镜设置中成功校准了仅相位的SLM响应.
    • 即使在低光成像条件下,也可以实现有效的校准.
    • 该技术证明了对强烈的光漂白效应的弹性.

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    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
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    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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    结论:

    • 这种基于图像的校准方法为非线性显微镜中仅相位的SLM提供了简单有效的解决方案.
    • 它大大降低了与SLM校准相关的复杂性和成本.
    • 这种方法适合在多光子光显微镜中常规使用,即使是要求高的样本.