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Bacterial Transformation

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...

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Updated: Jan 17, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

使用In-Cloning工具包进行模块化克隆 (MoClo) 的多基斯特朗操作子标准化模块化组合.

Jelmar de Vries¹, Timon A Lindeboom², Stijn T de Vries²

¹Laboratory of Systems and Synthetic Biology (SSB), Wageningen University and Research, Agrotechnology and Food Sciences.

Journal of visualized experiments : JoVE

|September 22, 2025

概括

本研究提出了一种使用模块化克隆 (MoClo) 工具包组装多基斯特龙操作子的新方法. 内外克隆工具包为合成生物学应用程序提供了复杂DNA分子的高效构建.

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Last Updated: Jan 17, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

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科学领域:

合成生物学合成生物学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

模块化克隆 (MoClo) 可快速组装多基因结构使用金门克隆与IIS型限制酶.
MoClo 通过使用标准化的悬架和防止切割连接的碎片,可以在单次反应中高效地组装DNA片段.
现有的细菌MoClo工具包主要侧重于单基斯特龙单元,缺乏多基斯特龙操作子组装的结构化方法.

研究的目的:

展示一个使用In- & Out-Cloning工具包组装多基斯特龙转录单元的协议.
提供一种可转移的方法,用于构建适用于各种MoClo系统的多基斯特龙操作子.
扩大MoClo工具包用于复杂的遗传电路设计的功能.

主要方法:

使用了In- & Out-Cloning工具包,这是模块化克隆 (MoClo) 的一种变体.
采用IIS型限制酶,用于具有特定突起的定向DNA组装.
组装多个DNA部分,包括促进子,RBS,编码序列和终止子,成为多基斯特朗转录单元.

主要成果:

成功演示了使用In- & Out-Cloning工具包组装多基斯特龙转录单元.
开发的协议允许高效和模块化的操作构建.
该方法被证明可以转移到其他MoClo工具包.

结论:

输入和输出克隆工具包提供了一个结构化的方法,用于组装多基斯特龙操作符.
该协议增强了MoClo在细菌中构建复杂遗传系统的实用性.
这些发现有助于先进的合成生物学应用,需要多基因基因表达.