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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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相关实验视频

Updated: Jan 17, 2026

Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells

Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells

Published on: January 21, 2021

超高分辨率成像使用纳米片.

Steffan Møller Sønderskov^1,2, Lasse Hyldgaard Klausen¹, Sebastian Amland Skaanvik^1,3

¹Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark.

|September 22, 2025

概括

此摘要是机器生成的。

超分辨率扫描离子导电显微镜 (SR-SICM) 通过克服分辨率限制和工件来增强纳米尺度成像. 这种新的技术可以在生理条件下进行例行单分子研究.

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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

相关实验视频

Last Updated: Jan 17, 2026

Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells

Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells

Published on: January 21, 2021

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

科学领域:

生物物理学的生物物理.
纳米技术纳米技术
显微镜的使用方法

背景情况:

了解纳米生物结构对于功能和动态至关重要.
现有的纳米级成像方法在分辨率和工件控制方面存在局限性.
扫描离子导电显微镜 (SICM) 具有潜力,但面临着解决方案的挑战.

研究的目的:

为SICM开发超分辨率技术,以改善结构成像.
克服传统SICM固有的分辨率限制和缺陷.
为了在纳米尺度上进行常规单分子研究.

主要方法:

开发了使用图像解卷处理的超分辨率SICM (SR-SICM).
利用模拟的管管点点差函数进行解卷.
将该技术应用于各种纳米结构.

主要成果:

SR-SICM显著超过了传统SICM的横向分辨率极限.
该技术有效地减轻了成像工件.
证明了对各种纳米结构的适用性.

结论:

SR-SICM提供了增强的纳米结构信息.
这款用户友好的软件有助于进行例行单分子成像.
这一进步对生物科学具有重大前景.