相关实验视频

Updated: Jan 17, 2026

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
11:47

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

Published on: August 1, 2016

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选择氨基酸-tRNA合成酶/tRNA对,以有效地将非正规氨基酸编码为蛋白质

Nathan D Alexander1, Yogesh M Gangarde1, Riley M Bednar1

  • 1GCE4All Research Center, Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR, USA.

Nature protocols
|September 22, 2025
PubMed
概括

这项研究提出了一个新的协议,有效地选择氨基酸-tRNA合成酶 (RS) /tRNA对用于遗传密码扩展. 该方法使得在各种表达系统中发现新型配对来结合非正规氨基酸 (ncAA).

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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells14:02

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