片IPVP和LPIA诱导胰岛素分泌在一个2层Caco-2-STC-1/BRIN-BD11细胞模型中
Maria Cermeño1, Marta Santos-Hernández1,2, Richard J FitzGerald1,3,4
1Department of Biological Sciences, University of Limerick, Castletroy, Limerick V94 T9PX, Ireland.
Journal of agricultural and food chemistry
|September 22, 2025
概括
来自酒厂消耗的谷物蛋白水解盐的两个,IPVP和LPIA,间接增强胰岛素分泌. 它们还增加了类似葡萄糖类-1 (GLP-1) 和抑制了二乙酶-IV (DPP-IV),这表明2型糖尿病的潜在管理.
科学领域:
- 生物化学 生物化学
- 内分泌学 在内分泌学.
- 营养科学 营养科学
背景情况:
- 酒厂的废谷物 (BSG) 是一个丰富的蛋白质来源.
- 蛋白质水解剂可以产生具有潜在健康益处的生物活性.
- 2型糖尿病的管理需要改善胰岛素分泌和葡萄糖控制的策略.
研究的目的:
- 为了研究IPVP和LPIA的胰岛素效应从BSG蛋白质水解衍生.
- 探索潜在的机制,包括GLP-1分泌和DPP-IV抑制.
- 评估这些的治疗潜力,治疗2型糖尿病.
主要方法:
- 使用了两层细胞模型:Caco-2/STC-1 (肠道/肠内分泌) 和BRIN-BD11 (胰腺β细胞).
- 从BRIN-BD11细胞中测量胰岛素分泌物,在对的顶部暴露时.
- 在试验室和现场评估GLP-1水平和二乙酶-IV (DPP-IV) 活性.
主要成果:
- 无论是IPVP还是LPIA,都间接地显著增强了BRIN-BD11细胞的胰岛素分泌.
- 在共同培养的细胞中,LPIA显著增加了GLP-1水平.
- IPVP和LPIA显示了强大的,非竞争性的混合型抑制DPP-IV活性,并抵抗了降解.
结论:
- IPVP和LPIA在2型糖尿病管理方面具有治疗潜力.
- 它们的机制包括增强GLP-1分泌和抑制DPP-IV.
- 需要进一步的分子和临床研究来验证它们的有效性.
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