在口腔致癌过程中增加的N6-甲基氨酸 (m6A) -RNA-甲基化
Zhiming Qin1,2, Yanting Chi1,2, Xinpei Wang1,2,3
1Department of Oral Pathology, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China.
概括
在口腔上皮质发育不良 (OED) 和口腔状细胞癌 (OSCC) 中,N6-甲基亚丁素 (m6A) -RNA甲基化升高,表明其在恶性转变中的作用. 超甲基化和C4B,DNAH9和NCALD的上调与更好的患者预后相关.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 口腔上皮质质变形 (OED) 和口腔状细胞癌 (OSCC) 涉及恶性转变期间的分子变化.
- 在OED和OSCC瘤发生过程中,N6-甲基氨酸 (m6A) -RNA甲基化的作用尚不清楚.
研究的目的:
- 从表观遗传学的角度研究OED和OSCC.
- 阐明口腔组织中恶性转变的分子机制.
- 确定m6A-RNA甲基化在OED和OSCC中的参与.
主要方法:
- 对OED和OSCC样本进行激光微剖切.
- 甲基化RNA免疫沉降测序 (MeRIP-Seq) 用于m6A修饰造型.
- 用于基因表达分析的RNA测序 (RNA-seq).
主要成果:
- 为OED和OSCC建立了全面的m6A-RNA甲基化和基因表达配置文件.
- 确定了四个超甲基化和11个低甲基化基因,这两种情况都很常见.
- 发现了107个上调和37个下调基因;C4B,DNAH9和NCALD显示过甲基化和上调,与更好的生存率相关.
结论:
- 与正常口腔上皮相比,OED和OSCC上皮组织中的m6A-RNA甲基化水平较高.
- m6A-RNA甲基化与OED到OSCC的发展和进展有关.
- 超甲基化和C4B,DNAH9和NCALD的上调与OED和OSCC的良好预后有关.
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