氨酸对维克拉莫密切斯异常体中乙醇压力的缓解作用
Yinfeng Li1, Yujie Wang2, Shuangyan Liu1
1College of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550000, China.
Journal of fungi (Basel, Switzerland)
|September 23, 2025
概括
补充氨酸可以通过减少氧化损伤和保持细胞完整性,在乙醇压力下提高酵母生存和发酵效率. 这项研究揭示了阿尔金宁.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 发酵技术的发酵技术
背景情况:
- 乙醇压力会损害酵母的生存能力和发酵效率.
- 氨酸是一种氨基酸,参与细胞应激反应.
研究的目的:
- 为了阐明氨酸在*Wickerhamomyces anomalus*中对乙醇压力的保护机制.
- 研究氨酸对细胞存活,氧化应激,膜完整性和氧化合成的影响.
主要方法:
- 在乙醇压力 (9%v/v) 下,用氨酸 (5mM) 补充 *W. anomalus*.
- 评估细胞存活率,活性氧物种 (ROS) 生产和使用光染色的膜完整性.
- 转录组学和代谢组学分析以确定差异表达基因 (DEG) 和代谢物 (DEM).
主要成果:
- 补充氨酸改善了细胞存活率并降低了ROS水平.
- 外源性氨酸维持了细胞和线粒体膜的完整性,并刺激了氧化的产生.
- 转录组和代谢组分析揭示了与酸盐代谢,化和脂质代谢相关的途径的调节.
结论:
- 氨酸在增强 *W. anomalus* 对乙醇压力的耐受性方面发挥着关键的机制作用.
- 研究结果表明,阿基尼因在酒精饮料发酵行业的潜在应用.
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