异质蛋白表达和天然产品合成的最新进展 通过Aspergillus
Yuyang Sheng1, Shangkun Qiu1, Yaoming Deng1
1College of Pharmacy, Shenzhen Technology University, Shenzhen 518118, China.
Journal of fungi (Basel, Switzerland)
|September 23, 2025
概括
类真菌是生产有价值化合物的强大的微生物工厂. 合成生物学和CRISPR-Cas9基因编辑的最新进展提高了它们在生产蛋白质和天然产品中的使用.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 合成生物学 合成生物学
背景情况:
- 丝状真菌属Aspergillus近一个世纪以来在工业上具有重要意义.
- 它用于生产有机酸,酶,重组蛋白质和天然化合物.
- 由于其蛋白质分泌,前体供应和翻译后修改能力,Aspergillus作为一个强大的异质底盘.
研究的目的:
- 审查最近在Aspergillus平台上的异质蛋白表达和天然产品生物合成方面的进展.
- 为了突出阿斯伯吉拉斯菌在类生物合成中的应用.
- 检查CRISPR-Cas9在Aspergillus niger和Aspergillus oryzae中的基因修饰的使用情况.
主要方法:
- 对最近的进展进行了全面的文献审查.
- 专注于在Aspergillus中的合成生物学应用.
- 对阿斯伯吉勒斯种类的CRISPR-Cas9基因编辑技术的分析.
主要成果:
- 阿斯伯吉路斯平台在异质蛋白表达和天然产品合成方面取得了显著进展.
- Aspergillus oryzae对于异质类生物合成特别有效.
- 克里斯普尔-Cas9技术已经成功地应用于阿斯伯吉illus niger和阿斯伯吉illus oryzae的遗传修饰.
结论:
- 阿斯伯吉勒斯种类是生物技术应用的非常有价值的平台.
- 继续开发Aspergillus表达系统对于天然产品生物合成具有很大的潜力.
- 克里斯普尔-Cas9增强了阿斯伯吉卢斯的基因工程能力,用于工业应用.
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