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miR-129-5p调节HMGB1/RAGE轴以抑制热和改善宫上皮细胞恶化

Huafang Wang¹, Dilidaer Sidike¹, Pan Liu¹

¹First Department of Oncology Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi.

European journal of histochemistry : EJH

|September 23, 2025

概括

微RNA-129-5p (miR-129-5p) 通过抑制HMGB1/RAGE介导的热和细胞损伤来抑制子宫癌的进展. 恢复miR-129-5p水平为宫癌提供了一个潜在的治疗策略.

科学领域:

妇科瘤学妇科瘤学
分子生物学分子生物学
细胞病理学细胞病理学

背景情况:

宫癌是一种严重的妇科恶性瘤,其分子机制尚不完全理解.
微RNA-129-5p (miR-129-5p) 在宫癌中调节细胞过程中的作用仍然在很大程度上未被阐明.

研究的目的:

调查miR-129-5p在高流动性组盒1 (HMGB1) /先进糖化最终产品 (RAGE) 受体轴上的调节机制.
为了确定miR-129-5p对热致死和宫上皮细胞恶化的影响.
探索miR-129-5p在宫癌中的治疗潜力.

主要方法:

定量实时PCR (RT-qPCR) 和西部涂抹来评估miR-129-5p和HMGB1的表达.
脂聚糖 (LPS) 刺激诱导宫上皮细胞恶化模型.
对miR-129-5p进行过度表达和淘汰实验,以评估其调节功能.
卡斯巴酶-1活性测试,EDU结合和殖民地形成测试以测量热,细胞活力和增殖.

主要成果:

宫癌细胞表现出显著下调的miR-129-5p和上调的HMGB1.1.
miR-129-5p的过度表达减少了HMGB1的表达,抑制了RAGE的激活,并降低了GSDMD-N (pyroptosis executer) 的产生.
由于miR-129-5p的过度表达,LPS诱导的热和细胞恶化被抑制,这也增强了细胞存活和增殖.

结论:

miR-129-5p通过抑制HMGB1/RAGE信号通路,在宫癌中起到瘤抑制作用.
过度表达miR-129-5p可以减轻火和细胞损伤,这表明它有可能成为治疗点.
这些发现为宫癌的发病和治疗策略提供了新的机制性见解.

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