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在SAMP8小鼠中的肠道微生物群动态:来自机器学习和纵向分析的见解

Yilang Ke¹, Aiping Zeng¹, Dang Li¹

¹Fujian Key Laboratory of Vascular Aging, Department of Geriatrics, Fujian Institute of Geriatrics, Fujian Clinical Research Center for Senile Vascular Aging and Brain Aging, Fujian Medical University Union Hospital, Fuzhou, Fujian, China.

Microbiology spectrum

|September 23, 2025

概括

衰老显著改变肠道微生物群,减少多样性和改变细菌组成. 这项研究确定了Peptococcus作为SAMP8小鼠衰老的潜在生物标志物.

科学领域:

微生物学微生物学
老年学是一门学科.
生物信息学是一种生物信息学.

背景情况:

肠道微生物群的组成随着年龄的增长而发生显著变化.
SAMP8小鼠模型表现出加速衰老,这使得它对研究与年龄相关的变化非常有价值.
对老化SAMP8小鼠肠道微生物群动态的纵向研究是有限的.

研究的目的:

在SAMP8小鼠的衰老过程中全面分析肠道微生物群的动态.
为了确定微生物多样性,组成和轨迹的与年龄相关的变化.
为了发现潜在的微生物生物标志物对衰老.

主要方法:

使用16S rRNA测序,在1,3,7和10个月的SAMP8小鼠中分析了肠道微生物群.
进行了α和β多样性分析,以评估微生物社区结构的变化.
随机森林分析被用于生物标志物发现,通过比较年轻和老年人群.

主要成果:

随着年龄的增长,阿尔法多样性 (香农指数) 显著下降.
贝塔多样性在年轻小鼠和老年小鼠之间显示出明显的集群.
关键的细菌系如Firmicutes和Actinobacteria的数量减少,而Proteobacteria和Bacteroidetes的数量增加.
球菌被确定为一个潜在的衰老生物标志物,具有很高的诊断准确性 (AUC = 0.78).

结论:

在SAMP8小鼠的老化过程中,肠道微生物群经历了显著的重组.
包括Peptococcus在内的特定细菌种群可以作为衰老的可靠生物标志物.
这些发现提供了关于微生物群与衰老相互作用的见解,以及干预的潜在目标.

关键词:

在SAMP8小鼠中.衰老的衰老衰老的衰老我们的肠道微生物群.纵向研究是一种纵向研究.机器学习是机器学习.

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