蛋白酶的高通量活动重编程 (HARP)
Samantha G Martinusen1, Ethan W Slaton1, Seyednima Ajayebi1
1Department of Chemical Engineering, University of Florida, Gainesville, Florida 32611, United States.
ACS chemical biology
|September 23, 2025
概括
蛋白酶的高通量活动重编程 (HARP) 平台有效地发现了强大的蛋白酶抑制剂. 这种基于酵母的屏隔离了抑制性宏分子,使得像TEVp这样的酶能够发现低纳米抑制剂.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 酶抑制剂的发现 酶抑制剂的发现
背景情况:
- 开发选择性蛋白酶抑制剂是具有挑战性的,因为难以分离罕见的,强大的分子.
- 现有的平台优先考虑高亲和度结合剂,需要广泛的下游表征.
研究的目的:
- 开发一种新的,高通量平台,用于发现蛋白酶抑制性宏分子.
- 隔离针对特定蛋白酶标的强效和选择性抑制剂.
主要方法:
- 开发了蛋白酶高通量活动重编程 (HARP) 平台,一种基于酵母的功能屏幕.
- 与可选择细胞表面表型的蛋白酶抑制相结合,用于大图书馆的高效选.
- 利用结构建模和深度测序进行抑制剂分析.
主要成果:
- 成功分离了低纳米度抑制纳米体,对抗烟草蚀刻病毒蛋白酶和人类kallikrein 6.
- 鉴定出一种罕见的10.5nMKI非竞争性TEVp抑制剂.
- 在抑制剂发现中,HARP展示了高动态范围和分辨率.
结论:
- 哈普 (HARP) 是一个主要的平台,用于发现对酶标的调节性宏分子.
- 该平台有助于从各种合成支架中分离出强效的抑制剂.
- 通过结构建模和深度测序获得了对抑制分子决定因素的洞察力.
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