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Updated: Jul 24, 2026

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines
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为PIK3CA H1047R突变检测建立潜在的参考测量程序和参考材料

Shangjun Wang1,2, Hongmei Ji2, Yuqian Jia3

  • 1State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 211189, P. R. China. gu@seu.edu.cn.

Analytical methods : advancing methods and applications
|September 23, 2025
PubMed
概括

开发了一种新的数字PCR方法和基因组参考材料,用于检测PIK3CA H1047R突变. 这提高了癌症诊断测试的准确性和质量控制,特别是乳腺癌.

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The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...