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时间解析的光蛋白在时间和光谱领域扩展光显微镜

Zizhu Tan¹, Chia-Heng Hsiung¹, Jiahui Feng²

¹Zhejiang Key Laboratory of Precise Synthesis of Functional Molecules, Department of Chemistry, Westlake University, 600 Dunyu Road, Hangzhou 310030, Zhejiang, China; Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou 310024, Zhejiang, China.

|September 23, 2025

概括

研究人员开发了新的时间解析光蛋白 (tr-FPs),以更好地了解细胞过程. 这些tr-FP可以在活细胞中进行更详细的成像和量化,从而促进生物研究.

科学领域:

生物物理
细胞生物学
分子成像

背景情况:

光显微镜是生命科学中的关键工具.
常态光强度是常用的,但时间解析 (tr) 信号,特别是光寿命,较少被探索.

研究的目的:

开发一种具有可调节寿命的新型定时光蛋白 (tr-FPs).
通过控制光寿命而不改变光谱特性,实现先进的成像技术.

主要方法:

在可见光谱中设计和合成了具有合理控制寿命的tr-FP家族.
使用时光光谱解析显微镜对活细胞进行同时多蛋白成像.
在多重化超分辨率显微镜中应用tr-FPs.

主要成果:

开发了具有广泛寿命的tr-FP,覆盖了可见光谱.
使用时光光谱解析显微镜在活细胞中实现9种不同蛋白质的同时成像.
通过超分辨率显微镜和量化细胞蛋白质立体测量,同时可视化4种蛋白质.

结论:

引入了tr-FP作为生物研究的变革性工具集.
证明了tr-FPs在整合系统复杂性和定量准确性的潜力.
开辟了先进细胞分析和理解生物机制的新途径.

关键词:

细胞周期铁症光的使用寿命光寿命显微镜多重成像氧化应激蛋白质固体测量量定量成像超分辨率显微镜时间溶解的光蛋白

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