分析元数据以探索与SARS-CoV-2和心血管疾病相互关联的枢纽基因的相互作用
Haily Liduin Koyou1, Vasudevan Ramachandran1,2,3, Mohd Nazil Salleh1
1Department of Medical Sciences, Faculty of Health Sciences, University College MAIWP International, Federal Territory of Kuala Lumpur, Malaysia.
Pneumologie (Stuttgart, Germany)
|September 23, 2025
概括
这项研究确定了关键基因,将SARS-CoV-2感染与心血管疾病 (CVD) 联系起来. 这些枢纽基因可以预测COVID-19后心血管疾病风险,并帮助早期检测病毒感染.
科学领域:
- 基因组学和生物信息学
- 传染性疾病 传染性疾病
- 心血管医学 心血管医学
背景情况:
- 感染SARS-CoV-2可以导致心脏损伤和随后的心血管疾病 (CVD).
- 确定COVID-19和心血管疾病之间的分子联系对于患者管理和风险预测至关重要.
研究的目的:
- 分析与SARS-CoV-2感染和心血管疾病之间高度相关的枢纽基因.
- 确定早期病毒感染检测和COVID-19后心血管疾病风险评估的潜在生物标志物.
主要方法:
- 利用转录基因数据集 (GSE196822,GSE196656,GSE169241) 进行使用DESeq2.2.的差异基因表达分析.
- 进行了通路丰富分析 (GO,KEGG) 并构建了蛋白质-蛋白质相互作用网络 (STRING,Cytoscape) 来识别枢纽基因.
- 使用qRT-PCR进行验证的顶部枢纽基因 (例如TIMP1,MPO,NFKBIA,ACSS1,OXCT1,ADHFE1).
主要成果:
- 确定了169个上调和123个下调的差异表达基因 (DEGs),这些基因与COVID-19和CVD共同存在.
- 凯格路径分析显示,癌症和代谢路径中转录错误调节的显著丰富.
- 顶级枢纽基因显示出显著的表达变化,10个基因中的9个显示出与COVID-19和心血管疾病并发症相关的高表达性.
结论:
- 确定了特定的枢纽基因 (例如,TIMP1,MPO,NFKBIA,ACSS1,OXCT1,ADHFE1) 作为SARS-CoV-2和心血管疾病之间的潜在分子联系.
- 这些枢纽基因可能作为病毒感染的早期指标和COVID-19后心血管疾病发病的预测因素.
- 推公共卫生查COVID-19并发症,特别是心血管疾病.
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