综合mRNA-seq和miRNA-seq分析显示,miR-210a-5p通过准RASL11B/Raf/MAPK通路来调节产卵母老化
Xiyu Zhao1, Xinyan Li1, Wenxin Zhang2
1State Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, and, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology , Sichuan Agricultural University, 611130, Sichuan, People's Republic of China.
Journal of animal science and biotechnology
|September 23, 2025
概括
的子宫衰老涉及一种新的途径,其中减少miR-210a-5p会增加RASL11B,激活MAPK信号,促进细胞衰老. 这一发现为维持母生殖健康提供了潜在的策略.
科学领域:
- 生殖生物学 生殖生物学
- 分子老年学分子学.
- 禽畜科学 禽畜科学 禽畜科学
背景情况:
- 子宫老化显著影响卵子质量和母的生殖性能.
- 在家禽中驱动子宫衰老的分子机制尚不清楚.
- 这项研究调查了与年龄相关的母子宫中的分子变化.
研究的目的:
- 为了表征基因表达和调节变化在老化子宫.
- 为了确定参与子宫衰老的关键分子通路.
- 阐明微RNA在子宫衰老中的作用.
主要方法:
- 来自不同年龄 (350,500,700天) 的母的子宫组织的转录和微RNA测序.
- 用于目标预测和途径识别的生物信息分析.
- 使用初级子宫上皮细胞和分子分析进行实验验证.
主要成果:
- 观察到细胞衰老基因的显著上调,包括p53通路,特别是在350至500天之间.
- 微RNA测序揭示了与年龄相关的miR-210a-5p的下调.
- miR-210a-5p直接针对RASL11B,其减少激活RASL11B-MAPK信号通路,促进子宫衰老表型和炎症.
结论:
- 在母子宫衰老中发现了一种涉及miR-210a-5p,RASL11B和MAPK信号的新型调节途径.
- 这一途径为与年龄相关的家禽繁殖下降提供了机械洞察力.
- 针对这种途径可以提供策略来保护子宫功能并延长母的生殖寿命.
相关概念视频
01:22MicroRNAs
3.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.8K
01:22MicroRNAs
23.9K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.9K
02:15Experimental RNAi
7.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.3K