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Updated: Jan 17, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

在Lactobacillus paracasei ATCC33434中使用内源型I-E CRISPR-Cas系统进行基因组编辑

Ni Zuo¹, Fangyuan Zuo², Yanqiang Liu²

¹Institute of Molecular Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Biotechnology and applied biochemistry

|September 24, 2025

概括

研究人员成功地在Lactobacillus paracasei ATCC334中使用了CRISPR-Cas基因编辑,使基因淘汰和集成成为可能. 这一突破允许设计有益的细菌,比如创造一种降解尼古丁的菌株.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
生物技术是生物技术.

背景情况:

乳酸菌 (Lactobacillus paracasei ATCC334) 是一种有益的细菌,在食品和健康领域具有应用.
有限的基因编辑技术阻碍了对L. paracasei ATCC334.的理解和利用.
有效的基因编辑对于释放这种益生菌菌株的全部潜力至关重要.

研究的目的:

为了探索和描述L. paracaseiATCC334.4中的内源型I-E集群定期间隔的短时间palindromic重复 (CRISPR) -Cas系统.
建立和验证L. paracaseiATCC334.4的基因编辑工具.
设计L. paracasei ATCC334用于新型应用,例如尼古丁降解.

主要方法:

对内源CRISPR-Cas系统的生物信息分析,包括重复,间隔和领导序列.
预测和验证原空间体相邻动图 (PAM).
通过等离子体和基因组干扰实验评估CRISPR-Cas系统切割活性和基因编辑效率.

主要成果:

在L. paracasei ATCC334.4.中对I-E型CRISPR-Cas系统的识别和表征.
成功验证了PAM识别和CRISPR-Cas系统活动.
使用开发的基因编辑工具,演示有效的基因淘汰和基因集成.
设计一种能够降解尼古丁的L. paracasei菌株.

结论:

这项研究成功地在L. paracasei ATCC334.4中建立了基于CRISPR-Cas的基因编辑.
这为基因操纵和乳酸菌的功能研究提供了一个强大的平台.
开发的方法为为各种工业和卫生应用工程乳酸菌铺平了道路.

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