相关实验视频

Updated: Jan 17, 2026

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

激活Piezo1和TRPV4通道有助于hCMEC/D3细胞的机械感应

Wenhan An^1,2, Min Sun¹, Xiulin Zhang³

¹Department of Rehabilitation Medicine, The Second Hospital of Shandong University, Jinan, China.

Frontiers in physiology

|September 24, 2025

概括

TRPV4和Piezo1通道是人类大脑内皮细胞中的关键机械传感器. 机械刺激激活这些通道,增加细胞内和释放ATP,这对血脑屏障 (BBB) 功能至关重要.

科学领域:

细胞生物学细胞生物学
神经科学是一个神经科学.
生物物理学的生物物理.

背景情况:

瞬态受体潜在化物4 (TRPV4) 和Piezo1通道是关键的机械传感器.
这些通道在血脑屏障 (BBB) 机械传感中的作用需要进一步研究.
人类大脑微血管内皮细胞 (hCMEC/D3) 作为人类BBB的体外模型.

研究的目的:

研究hCMEC/D3细胞中TRPV4和Piezo1通道的表达和功能.
确定TRPV4和Piezo1在人类BBB模型机械感应能力中的参与.

主要方法:

使用RT-PCR,免疫光和西部斑点进行TRPV4和PIEZO1mRNA和蛋白质表达的定量分析.
通过成像对机械刺激的反应对机械感应的功能评估.
使用通道特异性激动剂和抗剂,以及纯感受体阻断剂的药理学操纵.

主要成果:

在hCMEC/D3细胞的mRNA水平上,TRPV4和PIEZO1是最丰富的机械感知通道.
机械刺激 (冲刺,剪切应力) 显著增加了细胞内 ([Ca2+]i),而TRPV4和Piezo1抗剂减弱了这种作用.
激活TRPV4和PIEZO1释放腺三酸盐 (ATP),并阻断清素受体减少机械刺激诱导的[Ca2+]i.

结论:

TRPV4和PIEZO1通道在hCMEC/D3细胞中功能表达,并为BBB机械传感做出贡献.
机械刺激激活TRPV4和PIEZO1,导致流入和ATP释放,影响内皮细胞的反应.
这些发现凸显了TRPV4和PIEZO1在调节BBB机械传导中的重要性.

关键词:

这就是ATPATPATPATP.一个Piezo1频道.在 TRPV4 频道上播放.人类大脑的微血管内皮细胞机械感官传导传导是机械感官传导.

更多相关视频

Measurement of Vibration Detection Threshold and Tactile Spatial Acuity in Human Subjects

Measurement of Vibration Detection Threshold and Tactile Spatial Acuity in Human Subjects

Published on: September 1, 2016

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

相关概念视频

Mechanically-gated Ion Channels

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...

Tension Response at Adherens Junctions

Tension Response at Adherens Junctions

The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

G-Protein Gated Ion Channels

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision. The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...