益生菌Limosilactobacillus reuteri的完整基因组序列和基因组表征 PSC102
Ga-Yeong Lee1, Hae-Yeon Cho1, Syed Al Jawad Sayem1
1Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu, South Korea.
Open veterinary journal
|September 24, 2025
概括
一种猪便分离物Limosilactobacillus reuteri PSC102,具有有益的益生菌特性. 全基因组测序证实了其安全性和适用于人类和动物的适用性,缺乏毒性因子或抗生素耐药性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 肠道微生物群对于肠道健康和潜在的益生菌来源至关重要.
- 从猪便中分离出来的Limosilactobacillus reuteri PSC102,显示了对健康的好处.
研究的目的:
- 为了进行Limosilactobacillus reuteri的全基因组测序PSC102.2.
- 确定支持其益生菌潜力的分子特征.
主要方法:
- 使用PacBio-Illumina平台进行全基因组测序.
- 通过EzBioCloud,NCBI和Glimmer进行基因组装和解释.
- 泛基因组和核心基因组分析以评估功能性保护.
- 使用NCBI和BLAST识别抗菌素耐药性和毒性因素.
主要成果:
- L. reuteri PSC102 的基因组是一个单个圆形染色体 (2,048,626 bp),GC含量为38.9%.
- 1,846个蛋白质编码序列包括用于宿主微生物相互作用,耐压力和防御的基因.
- 该基因组包含2,446个泛基因组和1,222个核心基因组正统基因群,其中74个独特基因参与氨基酸和碳水化合物代谢.
- 没有检测到抗微生物耐药性基因或毒性因素.
结论:
- 基因组分析支持使用L. reuteri PSC102作为安全有效的益生菌.
- 它的分子特性验证了它在人类和动物健康中的应用.
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