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单细胞多组体数据显示,肝脏组织微环境的异质性是由高血压引起的

Hongfei Li^1,2, Lingyu Cui³, Murong Zhou³

¹Yangtze Delta Region Institute (Quzhou), University of Electronic Science and Technology of China, Quzhou 324003, China.

Molecular therapy. Nucleic acids

|September 24, 2025

概括

高血压 (高血压) 可能会损害肝脏. 这项研究确定了与高血压相关的特定肝细胞组和基因标记物,为肝脏疾病提供了潜在的早期指标.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
肝病学肝病学是一种肝病学.

背景情况:

已知高血压 (HTN) 可能会导致肝损伤,但其对不同肝细胞群体的具体影响尚不清楚.
识别这些细胞和分子变化可以为高血压和肝脏疾病提供早期生物标志物.

研究的目的:

通过使用先进的单细胞多组学技术,研究受高血压影响的肝脏组织中的微环境变化.
在特定的肝细胞亚群中识别与高血压相关的新型基因标记物和调控元素.

主要方法:

单细胞RNA测序 (scRNA-seq) 用于在正常和HTN肝脏组织中识别不同的肝细胞亚群.
用单细胞测定转化酶可访问的染色体 (scATAC-seq) 和基因素ChIP-seq来分析cis调节元件 (CREs) 的调节网络.

主要成果:

确定了一个特定的HTN相关的肝细胞亚群",肝细胞_1",含有潜在的致病基因 (UPB1,SDS,PCCA,CYP3A4,PPARGC1A).
一个涉及CYP3A4基因促进体的转录因子 (TF) 中介的调节网络被发现在HTN肝细胞中增加了其表达.
确定NR1H4和UPB1之间的增强剂介导的调节关系是肝脏代谢平衡的潜在调节者.

结论:

这项研究揭示了肝脏中新型高血压诱导的基因标记物和 cis-regulatory 元素.
这些发现为预防和治疗高血压相关的肝脏并发症提供了新的见解.

关键词:

MT:生物信息学在生物信息学方面.增强剂是一种增强剂.肝细胞肝细胞这种高血压,高血压.活动主办人活动主办人一个单细胞的单细胞.转录因子的转录因子

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