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选择正确的自动采样器瓶和插件,以提高质谱分析中的可复制性和准确性
Ashlee T Falls1, Erin S Baker1
1Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27514, USA.
概括
对于per-和多醇基物质 (PFAS) 分析,建议使用玻璃瓶和聚烯插件 (GP) 组合. 这样可以最大限度地减少来自蒸发和粘附的样本损失,提高数据可重现性.
科学领域:
- 分析化学 分析化学
- 环境科学 环境科学
- 材料科学 材料科学 材料科学
背景情况:
- 准确的分析数据依赖于适当的样品存储和处理.
- 自动采样器瓶和插件对于在质谱仪测量前的样品制备至关重要.
- 瓶子和插入材料 (玻璃,聚烯) 显著影响分析结果.
研究的目的:
- 为了评估瓶子和插入材料组合对和多醇基物质 (PFAS) 分析的影响.
- 评估数据可复制性,样本损失 (粘附和蒸发) 和物理特征的差异.
- 为了确定最佳的瓶子和插入组合,以进行可靠的PFAS测量.
主要方法:
- 测试了四种瓶/插头组合 (玻璃/玻璃,玻璃/聚烯,聚烯/玻璃,聚烯/聚烯).
- 在甲醇:水溶剂中对6种PFAS化合物进行了32天的分析.
- 测量了峰值区域的可重现性,粘附性和蒸发性,并在各种组合中进行了比较.
主要成果:
- 在第一天,高峰区域没有显著差异,但聚烯/聚烯的复制性较低 (>10% RSD).
- 在20°C下32天后,所有组合显示至少4个PFAS的峰值面积变化>10%.
- 玻璃插件表现出最多的附着性,而玻璃瓶显示出最少的蒸发.
结论:
- 粘附和蒸发都显著影响PFAS量化.
- 建议在PFAS分析中使用玻璃瓶/聚烯插件 (GP) 组合.
- GP将蒸发 (由于玻璃瓶) 和粘附 (由于聚烯插入) 降到最低,提高数据可靠性.
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