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[通过二维液态染色学-并联质谱法在有毒的生物样本中定量测定毒素]

Li Fang¹, Feng-Mei Qiu², Yu-Chao Wang¹

¹Zhoushan Municipal District Center for Disease Control and Prevention （Zhoushan Municipal Health Supervision Institute），Zhoushan 316021，China.

Se pu = Chinese journal of chromatography

|September 24, 2025

概括

海鲜中毒的四毒素 (TTX) 是一个严重的健康问题. 一种新的二维液态色谱-并联质谱 (2D-LC-MS/MS) 方法可以准确地检测人体样本中的TTX,有助于早期诊断和治疗.

科学领域:

分析化学分析化学
毒理学毒理学毒理学
生物化学生物化学

背景情况:

类毒素 (TTX) 是一种在某些海洋生物中发现的强有力的神经毒素,通过食用海鲜构成风险.
TTX中毒可能导致严重的神经肌肉和呼吸衰竭,突出需要快速临床诊断.
在生物样本中精确量化TTX对于评估中毒严重程度和患者预后至关重要.

研究的目的:

开发和验证一种敏感的方法,用于确定人体血和尿液中度的四毒素 (TTX).
为TTX中毒病例建立可靠的诊断工具,特别是与食用海鲜有关的病例.
提供一种方法,促进及时的医疗干预,并改善患者的治疗结果.

主要方法:

在TTX分析中采用了二维液态色谱-并联质谱 (2D-LC-MS/MS) 技术.
样品经过了精简的提取过程,然后在C18柱和胺柱上进行分离.
卡苏加辛被用作内部标准,通过矩阵匹配校准和选择反应监测 (SRM) 实现量化.

主要成果:

2D-LC-MS/MS方法在测试度范围内显示出出色的线性 (相关系数> 0.9994).
血和尿液的低检测极限 (LOD) 和量化极限 (LOQ) 分别为0.3和1.0μg/L.
高回收率 (84.4%-98.4%) 和较低的相对标准偏差 (RSD <7.5%) 证实了该方法的准确性和精度.

结论:

开发的2D-LC-MS/MS方法是准确的,快速的,并且需要最小的样品预处理.
这种经过验证的方法适用于在临床生物样本中可靠检测TTX.
该技术在食物中毒情景中成功识别了TTX,为法医和临床毒理学提供了宝贵的工具.

关键词:

生物样本是生物样本.质谱学 (MS) 是一种质谱学.血等离子体是什么?这是一种中毒,中毒.四极毒素 (TTX) 是一种毒素.两维液体色谱 (2D-LC) 是一种两维液体色谱.尿液尿液尿液

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