perfluorooctanoic 酸可以增加饮用水分配系统中的生物膜弹性和病毒性
Haibo Wang1, Yukang Li1, Hui Chen1,2
1Key Laboratory of Environmental Aquatic Chemistry, State Key Laboratory of Regional Environment and Sustainability, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Environmental science & technology
|September 24, 2025
概括
饮用水中的 perfluorooctanoic 酸 (PFOA) 促进生物膜的形成,并增加对消毒剂和抗生素的耐受性. 这种新出现的污染物对饮用水分配系统 (DWDS) 构成风险.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 公共卫生 公共卫生
背景情况:
- perfluorooctanoic 酸 (PFOA) 是饮用水分配系统 (DWDS) 中一个普遍的污染物.
- 对于PFOA对微生物生物膜的形成和DWDS中的消毒剂耐药性的影响尚不清楚.
研究的目的:
- 为了研究PFOA对DWDSs* Pseudomonas aeruginosa*生物膜形成的影响.
- 确定PFOA对生物膜耐受性对残留消毒剂和抗生素的影响.
- 阐明PFOA诱导的细菌弹性和毒性的变化背后的机制.
主要方法:
- 暴露Pseudomonas aeruginosa生物膜在流通和中层系统中的环境相关的PFOA度 (1-80 ng/L).
- 对生物膜形成,耐受性和抗生素最小抑制度 (MICs) 的评估.
- 对细菌基因表达,信号通路 (cAMP/Vfr) 和细胞外聚合物质 (EPS) 生产的分析.
- 使用真实的DWDS管道生物膜进行验证.
主要成果:
- PFOA显著促进了*Pseudomonas aeruginosa*生物膜的形成 (1.8至4.5倍),并增强了对的耐受性 (7至22倍).
- PFOA增加了对抗生素的生物膜耐药性 (MICs高25-67%) 和高调的毒性因子基因.
- 机制包括PFOA诱导的氧化应激,增强的定数感应,增加的EPS产量和激活的cAMP/Vfr信号.
结论:
- 在DWDS中暴露PFOA可以导致更有弹性和毒性细菌生物膜.
- 对消毒剂和抗生素的耐受性增加在饮用水中构成显著的微生物风险.
- 积极的风险评估对于解决DWDS中PFOA等新出现的污染物的间接影响至关重要.
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