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通过乳酸盐/GPR81信号通路调节动脉功能的有氧运动
Fengzhi Yu1,2, Yilan Guo1, Liang He3
1The Key Laboratory of Adolescent Health Assessment and Exercise Intervention of the Ministry of Education, East China Normal University, Shanghai, People's Republic of China.
有氧运动通过增加乳酸和激活GPR81/CREB/eNOS通路来改善肥胖,卵巢切除的小鼠的动脉功能. 这突出了运动对心血管健康的益处的新机制.
科学领域:
- 心血管生理学心血管生理学
- 内分泌学 在内分泌学.
- 代谢健康 代谢健康
背景情况:
- G蛋白结合受体81 (GPR81) 是一种乳酸受体,其在动脉功能中的作用基本未知.
- 肥胖和卵巢切除损害动脉健康,并与动脉样硬化有关.
- 乳酸盐 (LA) 是 GPR81.1. 的内源连接体.
研究的目的:
- 为了研究GPR81在调节动脉功能中的作用,在动脉健康受损的小鼠模型中.
- 在这个模型中阐明运动改善动脉功能的信号通路.
主要方法:
- 使用高脂肪饮食 (HFD) 和卵巢切除术 (OVX) 创建了一个小鼠模型,以诱导肥胖和动脉功能受损.
- 小鼠接受了为期8周的有氧运动干预.
- 使用Gpr81淘汰赛小鼠来确认GPR81.1.的作用.
- 评估了关键的分子标记物,包括乳酸盐,GPR81表达,p-CREB和eNOS.
主要成果:
- OVX和肥胖增加了动脉硬性,脂质不良,以及大动脉氧化低密度脂蛋白 (ox-LDL).
- 有氧运动逆转了这些不良影响,增加了血清乳酸和大动脉GPR81表达.
- 运动还增加了酸化cAMP反应元素结合蛋白1 (p-CREB) 和内皮氧化合成酶 (eNOS) 的表达.
- 在Gpr81淘汰赛小鼠中,运动未能改善动脉功能.
结论:
- 在卵巢切除的肥胖小鼠中,运动可以通过乳酸/GPR81/p-CREB/CREB/eNOS信号通路来增强动脉功能.
- GPR81对于调解运动对动脉健康的有益影响至关重要.
- 这一途径代表了改善心血管功能的潜在治疗标.
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