在更广泛的反应中,人类CD4+ T细胞识别了结核菌菌感染的巨细胞
Volodymyr Stetsenko1, Daniel P Gail1, Scott M Reba1
1Division of Infectious Diseases and HIV Medicine, Department of Medicine, University Hospitals Cleveland Medical Center, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
大多数Mycobacterium结核病 (Mtb) 特定的T细胞识别受感染的巨细胞,对于控制结核病 (TB) 至关重要. 针对疫苗中的第七类分泌系统 (T7SS) 基质可能会增强对结核病的保护.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 疫苗学 疫苗学 疫苗学
背景情况:
- CD4+ T 细胞反应对于控制结核病 (TB) 是至关重要的,因为它通过识别感染 Mycobacterium tuberculosis (Mtb) 的巨细胞来控制结核病 (TB).
- 然而,MTB特异性T细胞在多大程度上识别这些受感染的细胞尚不完全理解.
研究的目的:
- 量化MTB特异性CD4+T细胞的频率,以识别MTB感染的巨细胞在患有潜在结核病感染 (LTBI) 的人群中.
- 为了识别这些T细胞所准的抗原及其效应器功能.
- 为开发有效的结核病疫苗提供信息.
主要方法:
- 使用了感染mtb的单细胞衍生巨细胞和来自LTBI个体的自身记忆CD4+T细胞.
- 采用T细胞抗原受体 (TCR) 测序来分析T细胞克隆型.
- 进行抗原查,以识别T细胞识别的特定的Mtb抗原.
主要成果:
- >70%的独特和>90%的Mtb特异性T细胞受体 (TCR) 克隆类型的Mtb感染巨被识别.
- 一个T细胞子集需要外源抗原暴露,这表明识别不完全.
- 所有已识别的TCR都是针对第七类分泌系统 (T7SS) 基板的特异性.
- 特定于mtb的T细胞表现出效应因子功能,包括IFNγ,TNF,IL-2和GM-CSF的产生.
结论:
- 在LTBI中,大多数Mtb特异性T细胞识别受感染的巨细胞,主要向T7SS基质.
- 识别T7SS基质的T细胞显示了正规的效应器功能.
- 结核病疫苗诱导特定于T7SS基质的T细胞识别受感染的巨细胞可能会对结核病产生保护.
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