使用Trametes versicolor进行小麦的生物预处理,以在固态发酵中增强细胞质酶的生产
Anusuiya Singh1, Carolyn Palma Toloza2, María Victoria Riquelme Muñoz3
1Departamento Ingeniería Química y Ambiental, Universidad Técnica Federico Santa María, Santiago, Chile. anusuiyas.ngh@gmail.com.
Bioprocess and biosystems engineering
|September 24, 2025
概括
这项研究使用小麦的生物预处理与Trametes versicolor来增强纤维素含量和支持酶生产. 这种环保方法通过增加酶产量和回收有价值的寡糖化合物来改善生物炼油应用.
科学领域:
- 生物技术是生物技术.
- 生物质的价值化 生物质的价值化
- 酶工程是什么? 酶工程是什么?
背景情况:
- 小麦是生物乙醇和生物炼油厂的可再生资源,但其纤维素结构带来了挑战.
- 昂贵的预处理和昂贵的商业酶阻碍了有效利用.
- 开发可持续和具有成本效益的生物质加工方法至关重要.
研究的目的:
- 调查使用Trametes versicolor用于小麦的生物预处理的有效性.
- 提高纤维素含量,并通过固态发酵 (SSF) 促进酶生产.
- 评估从预处理的小麦中回收寡糖的潜力.
主要方法:
- 使用Trametes versicolor进行小麦的生物预处理.
- 通过酸性水解对预处理生物质成分 (纤维素,素) 的分析.
- 用Penicillium chrysogenum进行固体发酵 (SSF),用于细胞酶的生产.
- 测定乳糖酶和过氧化酶 (MnP) 活动.
- 通过酸水解检测氧化酸糖.
主要成果:
- 生物预处理将纤维素含量从36.24%提高到41.25%,并将红素从28.66%降低到21.12%.
- 预处理的小麦支持显著的细胞质生产 (FPU,BGL,CMC活动).
- 在第21天,氧化氧化糖在1.15g/L的度下被检测出来.
结论:
- "Trametes versicolor生物预处理是小麦的有效和环保方法.
- 将生物预处理与SSF相结合,可以提高生物炼油应用的酶产量.
- 这种综合方法为生物质价值化和寡糖回收提供了一个可持续的战略.
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