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一种新的肠炎病毒变体的完整基因组序列和生物特征,在UL2中删除了该变体

Huan Yang^1,2, Bing Zhang², Xiaoyue Yang²

¹Qingdao Agricultural University, 700 Changcheng Road, Chengyang District, Qingdao City, Shandong Province, 266109, China.

Archives of virology

|September 24, 2025

概括

在接种疫苗的子中发现了一种致病性肠炎病毒 (DEV) 菌株,DEV JS2024. 这种分离物可能是疫苗和野生型菌株之间的再组合的结果,由特定的基因删除表明.

科学领域:

兽医病毒学兽医病毒学
分子流行病学分子流行病学

背景情况:

肠炎病毒 (DEV) 在水禽中造成重大损失.
常规疫苗接种用于控制DEV爆发.
新型或改性病毒株的出现是一个持续的挑战.

研究的目的:

来自一个接种疫苗的农场的毒性DEV分离物 (DEV JS2024) 的特征.
为了研究DEV JS2024隔离物的毒性遗传基础.
在现场分离物中识别潜在的重组事件.

主要方法:

在DEV JS2024基因组的分离和测序.
用疫苗和毒性菌株进行比较基因组分析.
对受感染的子进行病理检查.

主要成果:

已经测序了DEV JS2024基因组 (161,600 bp).
与毒性菌株相比,UL2基因显示了528-bp的删除,但与减弱的疫苗菌株相同.
在其他病毒基因 (UL32,UL20,UL14,UL7,RS1,US5) 中发现了点突变.
感染导致了特征性的DEV病理和高病毒标位 (10^5 LD50/mL).

结论:

在UL2基因中的528-bp删除表明了重组事件.
这一事件可能发生在减弱的疫苗菌株和野生型毒性菌株之间.
这代表了首次报告在现场可能发生自然DEV重组的情况.

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