间歇性糖醇养形成了一个糖原储存表型,通过顺序脱化来增强酸盐的积累
Shi-Man Liang1, Jia-Qi Zhou1, Hai-Tian Xu1
1School of Engineering, Hangzhou Normal University, Hangzhou 310018, China.
Water research
|September 24, 2025
概括
间歇性糖养增强了废水处理中的化物产量,通过促进糖原储存和顺序脱,提高了去除效率.
科学领域:
- 环境微生物学环境微生物学
- 生物技术是生物技术.
- 污水处理 污水处理 污水处理
背景情况:
- 脱对于去除气至关重要,但对anammox工艺的有效酸盐积累具有挑战性.
- 了解像甘这样的替代碳来源的微生物机制是有限的.
研究的目的:
- 调查间歇性养方案是否与连续养相比增加了酸盐的积累.
- 为了阐明微生物和代谢机制驱动的酸盐积累使用糖.
主要方法:
- 经营的测序批量反应堆 (SBR) 间歇性料和上游无氧污泥毯 (UASB) 反应堆,在碳限制条件下连续料.
- 利用基因组解析的基因组学来分析微生物社区结构和基因丰富性.
- 进行表型分析以评估细胞内糖原含量和酶活性 (酸盐减少酶与酸盐减少酶).
主要成果:
- 间歇养的SBR实现了比连续养的UASB (18.9%) 显著更高的化物积累效率 (43.0%).
- 该SBR社区显示了糖原储存细菌的丰富,以及更多的糖原代谢基因.
- 现型分析显示,SBR中细胞内糖原和酸还原酶与酸还原酶活性比较高,表明了序列性脱化.
结论:
- 间歇性糖醇养促进了"顺序脱"的表型,通过引导代谢流向糖原储存.
- 这种养策略调节了酸盐和酸盐还原酶之间的电子分离,增强了酸盐的积累.
- 观察到的表型是通过养策略调节的塑性特征,为控制工程系统中的转化提供了一个新的范式.
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