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无细胞复合酶集成的布尔输出系统

Jingyao Chen¹, Aviva Borison¹, Douglas Densmore²

¹Department of Biomedical Engineering, Boston University, Boston, MA, USA.

|September 24, 2025

概括

我们开发了一个无细胞复合酶集成的布尔输出系统 (CRIBOS) 用于多重遗传电路. 这种便携式系统能够稳定地存储超过四个月的DNA信息,从而推进生物计算和生物制造应用.

科学领域:

合成生物学合成生物学
生物技术是生物技术.
基因工程是一种基因工程.

背景情况:

无细胞基因表达系统对于研究和生物制造至关重要.
现有的系统在复杂的遗传电路设计和可移植性方面存在局限性.

研究的目的:

引入无细胞复合酶集成布尔输出系统 (CRIBOS).
为了证明CRIBOS在无细胞环境中构建多重遗传电路的能力.
为了展示一个便携式的,基于纸张的CRIBOS用于生物信息存储.

主要方法:

开发了CRIBOS,这是一个基于特定站点的重组酶平台,用于无细胞多重遗传电路.
构建了超过20个多输入多输出电路,包括解码器.
基于全转录因子 (aTF) 的集成传感器用于环境传感.
使用纸质CRIBOS作为生物存储器.

主要成果:

通过使用CRIBOS成功构建和演示了20多个多重遗传电路.
通过将CRIBOS与aTF传感器相结合,实现了多重环境传感能力.
创建了一种基于纸张的CRIBOS设备,能够存储基于DNA的生物信息超过4个月.
证明了系统的可移植性,稳定性和低资源需求.

结论:

克里博斯将多重布尔逻辑门的应用扩展到无细胞系统.
该平台增强了遗传电路设计和应用的多功能性.
基于纸张的CRIBOS提供了一个稳定且便携式的生物信息存储解决方案.
这项工作为复杂的无细胞遗传电路开发开辟了新的途径.

关键词:

布尔式布尔式是一个布尔式.性转录因子的转录因子生物计算是生物计算的方法.没有细胞的细胞.环境传感环境传感遗传电路是一个遗传电路.合成生物学合成生物学

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