揭示性玉米粉作为Bacillus megaterium循环德克斯糖酶转移酶生物合成的有效基质:统计优化和生物化学见解
Izabella de Carvalho Batista Muniz1, Thaís Santos Moraes Lima1, Jéssica Ferreira Borges1
1Processes Engineering Laboratory, State University of Bahia Southwest, Itapetinga, Bahia, Brazil.
International journal of biological macromolecules
|September 24, 2025
概括
性玉米粉 (WCS) 显著增强了Bacillus megaterium.的循环德克斯特林糖酶转移酶 (CGTase) 生产. 这种具有成本效益的基质可以改善工业应用的酶产量和β-环氧素生产.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 工业微生物学 工业微生物学
背景情况:
- 循环二烯糖转移酶 (CGTase) 的高效生产对于循环二烯 (CD) 制造至关重要.
- 目前的方法在选择最佳发酵基质以最大限度地提高CGTase生物合成方面存在局限性.
研究的目的:
- 评估玉米粉 (WCS) 和箭根粉 (AS) 作为CGTase生产的替代基质.
- 使用Bacillus megaterium.优化发酵参数以获得最大CGTase产量.
- 在优化条件下产生的CGTase的生化特征.
主要方法:
- 使用WCS和AS作为基质的Bacillus megaterium进行水下发酵.
- 响应表面方法 (RSM) 与中央复合可旋转设计 (CCRD) 进行参数优化.
- 生物化学特征CGTase,包括热和pH稳定性测试.
主要成果:
- 玉米粉 (WCS) 在促进CGTase分泌方面被证明优于箭根粉 (AS).
- 在优化条件下 (3.07% WCS,0.5% 氨基酸,pH 7.56,60h,35°C) 产生最大的CGTase活性 (17.86 U/mL).
- 产生的CGTase表现出广泛的热和pH稳定性,在离子的存在下增强活性和热稳定性.
结论:
- 性玉米粉 (WCS) 是用于工业规模的CGTase和β-环氧素生产的高效和成本效益的基材.
- 使用WCS进行优化发酵,与传统基板相比,提供了显著的经济和运营优势.
- 这项研究为增强生物技术生产环氧素提供了有价值的见解.
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